Bordetella pertussis IgG and IgM with Reflex IB
Test Mnemonic
BPIMG
CPT Codes
- 86615 - QTY (2)
Includes
- B. pertussis IgG
- B. pertussis IgM
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Serum | SST (Gold) | Refrigerated | Separate serum from cells ASAP or within 2 hours of collection and transfer into standard aliquot tube. Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Please mark specimens plainly as 'acute' or 'convalescent.' |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.3 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | After separation from cells: 2 days |
| Refrigerated | After separation from cells: 2 weeks |
| Frozen | After separation from cells: 1 year |
Days Performed
Tue, Fri
Turnaround Time
2 - 6 days
Methodology
| Name | Description |
|---|---|
| Semi Quantitative Enzyme Linked Immunosorbent Assay |
Reference Range
| B. pertussis IgG | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Freetext | < or = 0.94 IV: Negative – No significant level of detectable B. pertussis IgG antibody | Freetext | 0.95-1.04 IV: Equivocal – Repeat testing in 10-14 days may be helpful | Freetext | > or = 1.05 IV: Positive – IgG antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis |
| B. pertussis IgM | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Freetext | < or = 0.9 IV: Negative - No significant level of detectable B. pertussis IgM antibody | Freetext | 1.0-1.1 IV: Equivocal - Repeat testing in 10-14 days may be helpful | Freetext | > or = 1.2 IV: Positive - IgM antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis |
Special Info
If Bordetella pertussis Antibody, IgG by ELISA is 1.05 IV or greater, then B. pertussis IgG Immunoblot testing will be added at an additional charge. If Bordetella pertussis Antibody, IgM by ELISA is 1.2 IV or greater, then Bordetella pertussis IgM Immunoblot will be added at an additional charge. Contaminated, heat-inactivated, or severely lipemic specimens are unacceptable. If possible, specimens from New York clients will be sent out to a New York DOH approved laboratory.
Clinical Info
CDC first-line tests for pertussis are PCR and/or culture. However, if serology is used to access late-stage pertussis, the recommended test is Bordetella pertussis Antibodies, IgA and IgG by ELISA with reflex to Immunoblot.