Borrelia burgdorferi Antibodies, Total by ELISA, CSF
Test Mnemonic
BBURGM
CPT Codes
- 86618 - QTY (1)
Aliases
- Lyme Antibodies, Total
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 3 mL | Cerebrospinal fluid (CSF) | Clean container | Refrigerated | Transfer 3 mL CSF to standard aliquot tube |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.5 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 8 hours |
| Refrigerated | 2 weeks |
| Frozen | 1 year (Avoid repeated freeze/thaw cycles) |
Days Performed
Sun - Sat
Turnaround Time
2 - 4 days
Methodology
| Name | Description |
|---|---|
| Semi Quantitative Enzyme Linked Immunosorbent Assay |
Special Info
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible. Heat-inactivated, contaminated or hemolyzed specimens are unacceptable.
Clinical Info
Use in conjunction with positive serologic testing for the workup of suspected acute Lyme neuroborreliosis. Do not order in the absence of clinical symptoms. The detection of antibodies to B. burgdorferi in cerebrospinal fluid may indicate central nervous system infection. However, consideration must be given to possible contamination by blood or transfer of serum antibodies across the blood-brain barrier. Current CDC recommendations for the serologic diagnosis of Lyme disease are to screen with a polyvalent ELISA test and confirm equivocal and positive results with immunoblot. Both IgM and IgG immunoblots should be performed on samples less than 4 weeks after appearance of erythema migrans. Only IgG immunoblot should be performed on samples greater than 4 weeks after the disease onset. IgM immunoblot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease. Please submit requests for appropriate immunoblot testing within 10 days. Once this test is performed, if: a) Negative - no further testing is done. b) Positive or equivocal - Immunoblot testing will be performed on the original sample upon receiving a request. Sample will be held for 30 days.