Colon Cancer Hotspot Gene Panel Tissue




Test Mnemonic

NGSCOL

CPT Codes

  • 81210 - QTY (1)
  • 81275 - QTY (1)
  • 81311 - QTY (1)
  • 81276 - QTY (1)

Aliases

  • NGS
  • NGSCOL
  • NGSCOLON

Includes

  • KRAS
  • NRAS
  • BRAF

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
10 mm squareBlock, Formalin fixed paraffinSlidesAmbient Formalin-fixed paraffin-embedded tissue slides. Transport and store slides at ambient temperature. 10 unstained section FFPE tissue on charged, unbaked slides plus one H&E stained section with best tumor area circled by a pathologist.

Stability

Environmental Condition Description
AmbientIndefinitely for FFPE slides. FFPE slides can be transported at ambient temperature.
RefrigeratedUnacceptable
FrozenUnacceptable

Days Performed

3 days per week

Turnaround Time

8 days

Methodology

Name Description
Next Gen Sequencing 
Next Generation DNA Sequencing 

Special Info

FNA specimens are preserved in CytoLyt solution and should be stored at 4C until DNA extraction can be performed. Transport to lab at ambient temperature is acceptable. Formalin-fixed paraffin-embedded tissue slides. Transport and store slides at ambient temperature. 10 unstained section FFPE tissue on charged, unbaked slides plus one H&E stained section with best tumor area circled by a pathologist.