FISH for RET (10q11)




Test Mnemonic

RET

CPT Codes

  • 88377 - QTY (1)

Aliases

  • RET

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
4 slidesParaffin block, formalin fixedSlidesAmbientAmbientPre and post H&E with four unstained slides

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
4 slides    Pre and post H&E with four unstained slides

Stability

Environmental Condition Description
AmbientFFPE slides room temperature

Days Performed

3 - 5 times per week

Turnaround Time

5 - 7 days

Methodology

Name Description
Fluorescent In-Situ Hybridization (FISH) 

Clinical Info

RET (ret proto-oncogene ) is located at chromosome 10q11.2, and encodes a receptor tyrosine kinase that is required for normal development, maturation, and maintenance of neural, neuroendocrine, kidney, and other tissue and cell types. RET rearrangements are present in approximately 1 – 2% of lung adenocarcinoma cases, and are more common in younger patients without a history of cigarette smoking. Rearrangements can involve multiple fusion partners, the most common of which is KIF5B. The RET-KIF5B fusion is generated by a somatic pericentric inversion of chromosome 10. RET fusions result in constitutive activation of the RET enzyme, leading to downstream signaling of pathways involved in cellular growth and differentiation. RET rearrangements may be associated with responsiveness to RET tyrosine kinase inhibitor therapy in patients with lung adenocarcinoma.

Clinical Reference

Drilon A, Wang L, Hasanovic A, et al. Response to Cabozantinib with RET fusion-positive lung adenocarcinomas. Cancer Discov 2013;3:630–5. National Comprehensive Cancer Network (NCCN) v6.2015