FISH Insight Analysis




Test Mnemonic

ISIGHT

CPT Codes

  • 88271 - QTY (2)
  • 88274 - QTY (2)
  • 88271 - QTY (3)

Aliases

  • InSight Prenatal Amnio FISH Chromosomes 13, 18, 21 and XY

Performing Laboratory

Integrated Genetics/Labcorp


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
5 mLFluid, amnioticSterile container Ambient 

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
3 mL     

Stability

Environmental Condition Description
Ambient5 days
Refrigerated5 days
FrozenUnacceptable

Days Performed

Mon - Fri

Turnaround Time

3 - 4 days

Methodology

Name Description
Fluorescent In-Situ Hybridization (FISH) 

Special Info

Do not centrifuge for any reason. It is standard of care that patients having InSight also have chromosome analysis performed to confirm InSight findings and to identify other abnormalities undetectable by InSight. Specimens which are frozen, low volume, hypocellular, submitted in fixative, glass containers or rubber stoppered tubes (rubber is toxic to amniocytes) will be rejected.

Clinical Info

Abnormality detected by ultrasound. Abnormal maternal serum screening results indicating increased risk for Down syndrome or trisomy 18. Late mid-trimester gestational age, i.e. >20-22 weeks. 3rd trimester high-risk pregnancy (to assist with delivery management decisions). Parental Robertsonian translocation involving chromosomes 13 or 21. Risk for X-linked disorder (sex determination). Because InSight provides only numeric information about chromosomes 13, 18, 21, X and Y, the results are preliminary. No irreversible therapeutic action should be initiated on the basis of InSight results alone. InSight cannot detect structural chromosome rearrangements, mosaicism, and other numeric abnormalities, which together account for one third of prenatally detectable chromosome abnormalities.