Maternal Serum Screen, First Trimester, hCG, PAPP-A, NT
Test Mnemonic
MATFIR
CPT Codes
- 81508 - QTY (1)
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 3 mL | Serum | SST (Gold) | Refrigerated | Patient Prep: Specimen must be drawn between 11 weeks, 0 days and 13 weeks, 6 days. (Crown-Rump length must be between 43 - 83.9 mm at time of specimen collection). Separate from cells ASAP or within 2 hours of collection and transfer into standard aliquot tube. Must submit Patient History for Maternal Serum Testing form with specimen. The following information is required with the order: Patient's date of birth, current weight, number of fetuses present, patient's race, if the patient has had a previous pregnancy with a trisomy, if the patient is currently smoking, if this is a repeat sample, the age of the egg donor if in vitro fertilization, the date of ultrasound, the crown-rump length (CRL) measurement, the nuchal translucency (NT) measurement and the name and certification number of the sonographer. NT must be measured when the CRL is between 38-83.9 mm. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 3 mL | Serum | No additive (Red) | Refrigerated | Patient Prep: Specimen must be drawn between 11 weeks, 0 days and 13 weeks, 6 days. (Crown-Rump length must be between 43 - 83.9 mm at time of specimen collection). Separate from cells ASAP or within 2 hours of collection and transfer into standard aliquot tube. Must submit Patient History for Maternal Serum Testing form with specimen. The following information is required with the order: Patient's date of birth, current weight, number of fetuses present, patient's race, if the patient has had a previous pregnancy with a trisomy, if the patient is currently smoking, if this is a repeat sample, the age of the egg donor if in vitro fertilization, the date of ultrasound, the crown-rump length (CRL) measurement, the nuchal translucency (NT) measurement and the name and certification number of the sonographer. NT must be measured when the CRL is between 38-83.9 mm. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Refrigerated | After separation from cells: 2 weeks |
| Frozen | After separation from cells: 1 year (Avoid repeated freeze/thaw cycles) |
| Ambient | After separation from cells: 72 hours |
Days Performed
Sun - Sat
Turnaround Time
3 - 5 days
Methodology
| Name | Description |
|---|---|
| Quantitative Chemiluminescent Immunoassay |
Reference Range
Special Info
Patient Prep: Specimen must be drawn between 11 weeks, 0 days and 13 weeks, 6 days. (CRL must be between 43 - 83.9 mm at time of specimen collection). Must submit Patient History for Maternal Serum Testing form with the specimen. The following information is required with the order: Patient's date of birth, current weight, number of fetuses present, patient's race, if the patient has had a previous pregnancy with a trisomy, if the patient is currently smoking, if this is a repeat sample, the age of the egg donor if in vitro fertilization, the date of ultrasound, the CRL measurement, the nuchal translucency (NT) measurement and the name and certification number of the sonographer. NT must be measured when the CRL is between 38 - 83.9 mm. Plasma or hemolyzed specimens are unacceptable. This test is New York DOH approved.
Clinical Info
Used as a first-trimester screening test for trisomy 21 (Down syndrome) and trisomy 18. Does not include alpha fetoprotein for open neural tube defects. Requires nuchal translucency measurement performed by an ultrasonographer certified by the Fetal Medicine Foundation (FMF) or Nuchal Translucency Quality Review (NTQR).