Melanoma Hotspot Gene Panel Tissue
Test Mnemonic
NGSMEL
CPT Codes
- 81272 - QTY (1)
- 81311 - QTY (1)
- 81210 - QTY (1)
Aliases
- Next Generation Sequencing Hotspot Melanoma
- NGS
- NGSMEL
Includes
- KIT
- BRAF
- NRAS
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 10 mm square | Block, Formalin fixed paraffin | Slides | Ambient | Ambient | Formalin-fixed paraffin-embedded tissue slides. Transport and store slides at ambient temperature. 10 unstained sections FFPE tissue on charged, unbaked slides plus one H&E stained section with best tumor area circled by a pathologist. |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Ambient: Indefinitely for FFPE slides. FFPE slides can be transported at ambient temperature. |
| Refrigerated | Unacceptable |
| Frozen | Unacceptable |
Days Performed
3 days per week
Turnaround Time
8 days
Methodology
| Name | Description |
|---|---|
| Next Gen Sequencing | |
| Next Generation DNA Sequencing |
Special Info
FNA specimens are preserved in CytoLyt solution and should be stored at 4C until DNA extraction can be performed. Transport to lab at ambient temperature is acceptable. Formalin-fixed paraffin-embedded tissue slides. Transport and store slides at ambient temperature. 10 unstained sections FFPE tissue on charged, unbaked slides plus one H&E stained section with best tumor area circled by a pathologist.