MSI (PCR) X 2
Test Mnemonic
MSICCT
CPT Codes
- 81301 - QTY (1)
Aliases
- Microsatellite Instability (MSI) x 2
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| Other | Other | Other | Representative tumor: formalin-fixed, paraffin-embedded block, OR 10 unstained slides | ||
| Other | Other | Other | Representative patient normal: formalin-fixed, paraffin-embedded block, 5 unstained slides, preferably from separate block |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 4 mL | Whole blood | EDTA (Lavender) | Ambient | Ambient | Representative patient normal: Whole blood if no FFPE tissue is available (e.g. small biopsies) |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Whole Blood- 48 hours; Paraffin-Embedded tissue- indefinitly |
| Refrigerated | Whole blood- 7 days |
| Frozen | unacceptable |
Days Performed
2 days per week
Turnaround Time
7 days
Methodology
| Name | Description |
|---|---|
| Capillary Electrophoresis (CE) | |
| Polymerase Chain Reaction (PCR) |
Special Info
Please order this procedure when there are two specimens. Submit tissue specimen with anatomic pathology request form, and indicate MSI on the form. Histopathologic review will be performed. DNA mismatch repair immunohistochemistry may be added on to or substituted for MSI testing. BRAF mutation testing will be performed on all MSI-high, MLH1 loss cancers.
Clinical Info
Screening test for Hereditary Non-Polyposis Colorectal Cancer (HNPCC)