Platelet Antibody Detection
Test Mnemonic
PLTDET
CPT Codes
- 86022 - QTY (1)
LOINC ®
24375-8
Aliases
- Platelet Antibodies, Indirect
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Serum | SST (Gold) | Frozen | Separate serum from cells ASAP or within 2 hours of collection, aliquot and freeze. For newborns < 30 days old, collect specimen from the mother. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.5 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Unacceptable |
| Refrigerated | 48 hours |
| Frozen | 1 month |
Days Performed
Mon - Sat
Turnaround Time
2 - 4 days
Methodology
| Name | Description |
|---|---|
| Enzyme-Linked Immunosorbent Assay (ELISA) |
Reference Range
Special Info
Unacceptable Conditions: Microbial contamination, hemolyzed, lipemic, or heat inactivated samples. Testing for neonatal alloimmune thrombocytopenia should be performed using a maternal serum, since platelet antibody may not be detected in a neonatal serum. False negative results are common in infant samples. This test is New York DOH approved.
Clinical Info
This is the primary test for detection of platelet-specific antibodies. It is not recommended for the diagnosis of immune thrombocytopenic purpura (ITP). This test will detect both allo and autoantibodes, but will not distinguish between them. Results of this test should be used in conjunction with clinical findings and other serological tests. Antibodies directed to antigens found on platelets are associated with many different clinical situations. Immune thrombocytopenic purpura (ITP) is a destructive thrombocytopenia caused by autoantibodies. Neonatal alloimmune thrombocytopenia (NATP) and post-transfusion purpura (PTP) are diseases where thrombocytopenia is caused by platelet-specific alloantibodies. HLA alloantibodies do not cause thrombocytopenia, but are commonly associated with refractoriness to platelet transfusions. This test is designed to detect antibodies to platelet glycoproteins IIb/IIIa (HPA-1a/1b [P1A1 and P1A2], HPA-3a/3b, and HPA-4a), Ia/IIA (HPA-5a/5b), Ib/IX, and IV. In addition, this test will also detect antibodies to HLA Class 1 antigens (HLA-A-B).