Aldosterone/Direct Renin Ratio
Test Mnemonic
ALDREN
CPT Codes
- 84244 - QTY (1)
- 82088 - QTY (1)
Includes
- Aldosterone
- Renin
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 mL | Plasma | EDTA (Lavender) | Ambient | Frozen, Critical | Centrifuge and remove plasma from cells within 4 hours of collection. Freeze plasma immediately after separation from cells. |
Stability
| Environmental Condition | Description |
|---|---|
| Frozen | 30 days |
| Ambient | Unacceptable |
| Refrigerated | Unacceptable |
Days Performed
Mon - Fri
Turnaround Time
2 - 7 days
Methodology
| Name | Description |
|---|---|
| Chemiluminescence Immunoassay (CLIA) |
Special Info
Fasting specimens are recommended but not required. Record the time of day and patient's posture during blood collection (supine or upright). DO NOT pre-chill collection tubes, store tubes on ice or refrigerate; cryoactivation of prorenin occurs when samples are refrigerated. Biotin levels of up to 100 mg/day have not shown interference with this assay. Patients taking >100 mg/day to 300 mg/day should refrain from taking Biotin for 1 hour prior to sample collection. Patients taking a Biotin dose >300 mg/day should consult with their physician or the laboratory prior to having a sample taken. Clinicians should consider biotin interference as a source of error, when clinically suspicious of the laboratory result.
Clinical Info
Diagnosis of disorders of the renin-angiotensin-aldosterone system. An increased Aldo/Renin ratio is suggestive, but not diagnostic of primary aldosteronism if aldosterone concentration is > 15 ng/dL. Normal serum levels of aldosterone are dependent on the sodium intake and whether the patient is upright or supine. High sodium intake will tend to suppress serum aldosterone, whereas low sodium intake will elevate serum aldosterone. The reference interval for serum aldosterone are based on normal sodium intake.
Clinical Limitation
Criteria for Rejection: Refrigerated, grossly hemolyzed, grossly lipemic, and obviously microbially contaminated samples will be rejected. Interfering substances: Prorenin