Histocompatibility & Immunogenetics

Pathology Consultative Services

Histocompatibility & Immunogenetics

Allogen Laboratories—a department within Cleveland Clinic’s Pathology & Laboratory Medicine Institute—features more than 50 years of expertise in human leukocyte antigen (HLA) typing, crossmatching, and antibody determination.

Services include a complete testing menu and expert guidance for solid organ and hematopoietic stem cell transplant programs supporting pre- and post-transplant patient needs.

Have a question or need assistance? Contact Allogen Laboratories at 216.444.6582 or coordinator@ccf.org.

Histocompatibility & Immunogenetics Services

Human Leukocyte Antigen (HLA) Typing  |  Crossmatching  |  Antibody Determination

Solid Organ Transplant Support – Hematopoietic Stem Cell Transplant Support – Molecular Testing

Comprehensive testing provides all of the information necessary to assist in a positive transplant outcome.

Allogen Laboratories is fully licensed and accredited by The American Society for Histocompatibility and Immunogenetics (ASHI), the College of American Pathologists (CAP), and certified according to the Clinical Laboratory Improvement Amendments (CLIA).

Specimen Information

Collection & Transport

Do not refrigerate blood specimens.

Send samples immediately after drawing to be received by Allogen Laboratories within 24 hours of collection.

Send Specimens to:

Cleveland Clinic
Allogen Laboratories – C100
10524 Euclid Ave
Cleveland, OH 44106

Contact Allogen Laboratories

216.444.6582

216.444.8261 (fax)

coordinator@ccf.org

Specimen Requirements

Initial Workup, Solid Organ recipient/donor

Specimen Type
Whole blood

Volume
6 mL (x3)
4 mL (x1)

Collection Tubes
(3) Red (No Additive) Serum Tubes, and
(1) Lavender K2EDTA Tube

Initial Workup, Bone Marrow recipient/donor

Specimen Type
Whole blood

Volume
4 mL

Collection Tube
(1) Lavender K2EDTA Tube

Engraftment / Chimerism

Specimen Type
Whole blood

Volume
10 mL

Collection Tube
(1) Yellow Glass Whole Blood Acid Citrate Dextrose (ACD) Tube

Crossmatch

Patient

Specimen Type
Whole blood

Volume
6 mL

Collection Tube
(1) Red (No Additive) Serum Tube

Donor

Specimen Type
Whole blood

Volume
10 mL (x4)

Collection Tube
(4) Green Sodium Heparin Tubes

Antibody Testing

Specimen Type
Whole blood

Volume
6 mL (x3)

Collection Tube
(3) Red (No Additive) Serum Tube

Other

Please contact Allogen Laboratories.

Histocompatibility & Immunogenetics Test Menu

Flow Crossmatch

Test Name

XM T & B Flow ALLO/Auto

XM T & B Flow ALLO/Auto – Additional Serum Sample

CPT Code

86825

86826

Molecular Testing

Test Name

HLA-A,B,Cw,DR,DQ,DP,DRB345 – NGS, High Resolution results

HLA-A,B,Cw,DR,DQ,DP,DRB345 – NGS, Low Resolution results

HLA-A,B,Cw,DR,DQ,DP,DRB345 – rSSOP, Low Resolution results

CPT Code

81378

81370

81370

STEM CELL Chimerism, Genotyping

STEM CELL Chimerism, Follow up Assay with Sorted T-cells

Stem Cell Chimerism NGS, Genotyping

Stem Cell Chimerism NGS, Follow up Assay with Assorted T-cells

81265

81267

81479

81479

Solid Organ Chimerism Testing, STR-Genotyping

Solid Organ Chimerism Testing, STR

81265

81267

Antibody Testing

Test Name

Ab PRA Flow

CPT Code

86828

Ab ID Class I Luminex

Ab ID Class II Luminex

86832

86833

Ab ID Class I Luminex, Dilution

Ab ID Class II Luminex, Dilution

86832

86833

Ab Post-TX DSA Monitoring Class II

Ab Post-TX DSA Monitoring Class I

86832

86833

Class I Luminex Phenotype Beads

Class II Luminex Phenotype Beads

86832

86833

Ab ID Class I Luminex – C1q

Ab ID Class II Luminex – C1q

86832

86833

AT1R antibody determination

AT1R antibody determination, reference sample

83516

83516

Non-HLA Auto Antibody

Non-HLA Auto Antibody, reference sample

83516

83516

Additional Services

Test Name

Specimen Acquisition: Kit for Daily Testing

CPT Code

99001

Contact – Allogen Laboratories

216.444.6582

216.444.8261 (fax)

For general laboratory questions, please contact Client Services.

Fast Results

Turnaround for most antibody testing is within 48 hours.

Expert Consultation

Our staff is available for consultation during any stage of diagnosis or treatment.

Flexible Billing Solutions

Options include third-party and client billing that can be customized to your needs.

Reporting Options

Concise, in-depth reports are available for each consultation.

Histocompatibility & Immunogenetics Staff

Aiwen Zhang, PhD

Aiwen Zhang, PhD
Director, Histocompatibility & Immunogenetics

Ray Jurcago BS, CHS

Ray Jurcago, BS, CHS
Laboratory Manager