POLE Mutation Detection by Droplet Digital PCR
Test Mnemonic
POLEDD
CPT Codes
- 81479 - QTY (1)
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 10 mm square | Block, Formalin fixed paraffin | Formalin-fixed, paraffin-embedded block | Ambient | Ambient | Formalin-fixed paraffin embedded tissue slides. Transport and store slides at ambient temperature. Ten unstained sections FFPE on charged, unbaked slides or scrolls plus one H&E stained section with best tumor area circled by a pathologist. Provide percentage of tumor cells present, minimum 10%. Specimens that undergo heavy metal fixation or are decalcified with strong acids (i.e., formic acid) are not acceptable for molecular testing. Non-decalcified specimens or samples that undergo EDTA decalcification are acceptable. |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | FFPE samples collected and stored at ambient temperature |
| Refrigerated | Refrigerated FFPE samples may be accepted |
| Frozen | Frozen FFPE samples will be rejected |
Days Performed
2 days per week
Turnaround Time
5 days
Methodology
| Name | Description |
|---|---|
| Droplet Digital Polymerase Chain Reaction (PCR) |
Clinical Reference
Ten unstained sections FFPE on charged, unbaked slides or scrolls plus one H&E stained section with best tumor area circled by a pathologist. Provide percentage of tumor cells present, minimum 10%.