Testosterone, Free and Total, by Equilibrium Dialysis and Mass Spectrometry

Testosterone, Free and Total, by Equilibrium Dialysis and Mass Spectrometry Print

Test Mnemonic

TESTTF

CPT Codes

84402 - QTY (1)
84403 - QTY (1)

LOINC ^®

58952-3

Performing Laboratory

Cleveland Clinic Laboratories

Specimen Requirements

Volume	Type	Container	Collect Temperature	Transport Temperature	Special Instructions
1.8 mL	Serum	Red Plain		Refrigerated	Allow specimen to clot. Centrifuge and transfer the serum to a plastic CCL tube within 2 hours of collection and refrigerate.

Minimum Specimen Requirements

Volume	Type	Container	Collect Temperature	Transport Temperature	Special Instructions
1 mL

Stability

Environmental Condition	Description
Refrigerated	14 days
Ambient	3 days
Frozen	30 days

Turnaround Time

2 - 5 days

Methodology

Name	Description
Equilibrium Dialysis
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reference Range

Free Testosterone
Sex	Age From	Age To	Type	Range	Range Unit
Female	Days	364 Days	Normal	<0.10	ng/dL
Female	1 Years	5 Years	Normal	<0.10	ng/dL
Female	6 Years		Normal	<0.10	ng/dL
Female	7 Years		Normal	<0.10	ng/dL
Female	8 Years		Normal	<= 0.14	ng/dL
Female	9 Years		Normal	<= 0.18	ng/dL
Female	10 Years		Normal	<= 0.24	ng/dL
Female	11 Years		Normal	<= 0.29	ng/dL
Female	12 Years		Normal	<= 0.34	ng/dL
Female	13 Years		Normal	<= 0.38	ng/dL
Female	14 Years		Normal	<= 0.42	ng/dL
Female	15 Years	18 Years	Normal	<= 0.44	ng/dL
Female	19 Years	24 Years	Normal	<= 0.43	ng/dL
Female	25 Years	29 Years	Normal	<= 0.42	ng/dL
Female	30 Years	34 Years	Normal	<= 0.41	ng/dL
Female	35 Years	39 Years	Normal	<= 0.40	ng/dL
Female	40 Years	44 Years	Normal	<= 0.39	ng/dL
Female	45 Years	49 Years	Normal	<= 0.38	ng/dL
Female	50 Years	54 Years	Normal	<= 0.37	ng/dL
Female	55 Years	59 Years	Normal	<= 0.36	ng/dL
Female	60 Years	64 Years	Normal	<= 0.35	ng/dL
Female	65 Years	69 Years	Normal	<= 0.34	ng/dL
Female	70 Years	74 Years	Normal	<= 0.33	ng/dL
Female	75 Years	79 Years	Normal	<= 0.32	ng/dL
Female	80 Years	84 Years	Normal	<= 0.30	ng/dL
Female	85 Years	89 Years	Normal	<= 0.29	ng/dL
Female	90 Years	94 Years	Normal	<= 0.28	ng/dL
Female	95 Years		Normal	<= 0.27	ng/dL
Male	Days	364 Days	Normal	<= 1.24	ng/dL
Male	1 Years	8 Years	Normal	<0.10	ng/dL
Male	9 Years		Normal	<= 0.18	ng/dL
Male	10 Years		Normal	<= 0.50	ng/dL
Male	11 Years		Normal	<= 2.21	ng/dL
Male	12 Years		Normal	<= 3.94	ng/dL
Male	13 Years		Normal	<= 5.68	ng/dL
Male	14 Years		Normal	0.19 - 7.09	ng/dL
Male	15 Years		Normal	0.65 - 8.35	ng/dL
Male	16 Years		Normal	1.17 - 9.29	ng/dL
Male	17 Years		Normal	1.71 - 10.02	ng/dL
Male	18 Years		Normal	2.16 - 10.49	ng/dL
Male	19 Years		Normal	2.14 - 10.18	ng/dL
Male	20 Years	24 Years	Normal	2.10 - 9.92	ng/dL
Male	25 Years	29 Years	Normal	2.02 - 9.45	ng/dL
Male	30 Years	34 Years	Normal	1.94 - 9.03	ng/dL
Male	35 Years	39 Years	Normal	1.86 - 8.56	ng/dL
Male	40 Years	44 Years	Normal	1.78 - 8.03	ng/dL
Male	45 Years	49 Years	Normal	1.70 - 7.67	ng/dL
Male	50 Years	54 Years	Normal	1.62 - 7.25	ng/dL
Male	55 Years	59 Years	Normal	1.55 - 6.78	ng/dL
Male	60 Years	64 Years	Normal	1.47 - 6.36	ng/dL
Male	65 Years	69 Years	Normal	1.39 - 5.89	ng/dL
Male	70 Years	74 Years	Normal	1.31 - 5.47	ng/dL
Male	75 Years	79 Years	Normal	1.23 - 5.00	ng/dL
Male	80 Years	84 Years	Normal	1.15 - 4.58	ng/dL
Male	85 Years	89 Years	Normal	1.08 - 4.12	ng/dL
Male	90 Years	94 Years	Normal	1.00 - 3.67	ng/dL
Male	95 Years		Normal	0.92 - 3.23	ng/dL
Total Testosterone
Sex	Age From	Age To	Type	Range	Range Unit
Female	Days	14 Days	Normal	1.7 - 13.4	ng/dL
Female	15 Days	364 Days	Normal	<= 6.3	ng/dL
Female	1 Years	12 Years	Normal	1.7 - 32.3	ng/dL
Female	13 Years	15 Years	Normal	14.4 - 39.2	ng/dL
Female	16 Years	18 Years	Normal	3.2 - 66.6	ng/dL
Female	19 Years		Normal	8.0 - 60.0	ng/dL
Male	Days	14 Days	Normal	7.2 - 183.0	ng/dL
Male	15 Days	364 Days	Normal	1.4 - 257.0	ng/dL
Male	1 Years	12 Years	Normal	1.7 - 32.3	ng/dL
Male	13 Years	15 Years	Normal	28.0 - 453.0	ng/dL
Male	16 Years	18 Years	Normal	27.7 - 674.0	ng/dL
Male	19 Years		Normal	240.0 - 950.0	ng/dL

Special Info

Free testosterone is directly measured after equilibrium dialysis by liquid-chromatography and tandem mass spectrometry (LC-MS/MS).Â

Clinical Info

This test measures testosterone and direct free testosterone concentrations using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and equilibrium dialysis (ED). It may be used to assess patients with suspected increased or decreased biologically active testosterone, including those with sex hormone-binding protein abnormalities, cisgender women, children, cisgender men with hypogonadism, and for monitoring patients on testosterone-suppressing therapy.

Clinical Limitation

Tanner Stage reference intervals are not yet established.Â