Vitamin B3/Niacin




Test Mnemonic

B3VIT

CPT Codes

  • 84591 - QTY (1)

Aliases

  • Nicotinic Acid

Includes

  • Nicotinic Acid (Niacin)
  • Nicotinamide
  • Nicotinuric Acid

Performing Laboratory

ARUP


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mLSerumNo additive (Red) FrozenPatient should fast for 4 hours prior to collection and avoid the use of vitamin supplements for 24 hours prior to collection. Protect specimen from light during storage and shipment. Separate serum from cells ASAP or within 2 hours of collection and freeze. Use amber transport tubes.

Alternate Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mLSerumSST (Gold) FrozenPatient should fast for 4 hours prior to collection and avoid the use of vitamin supplements for 24 hours prior to collection. Protect specimen from light during storage and shipment. Separate serum from cells ASAP or within 2 hours of collection and freeze. Use amber transport tubes.
1 mLPlasmaSodium or Lithium heparin (Green) FrozenPatient should fast for 4 hours prior to collection and avoid the use of vitamin supplements for 24 hours prior to collection. Protect specimen from light during storage and shipment. Separate plasma from cells ASAP or within 2 hours of collection and freeze. Use amber transport tubes.
1 mLPlasmaLithium heparin PST (Lt. Green) FrozenPatient should fast for 4 hours prior to collection and avoid the use of vitamin supplements for 24 hours prior to collection. Protect specimen from light during storage and shipment. Separate plasma from cells ASAP or within 2 hours of collection and freeze. Use amber transport tubes.

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
0.3 mL     

Stability

Environmental Condition Description
AmbientAfter separation from cells: 6 hours
RefrigeratedUnacceptable
FrozenAfter separation from cells: 1 month

Days Performed

Sun, Tue, Wed, Fri, Sat

Turnaround Time

2 - 6 days

Methodology

Name Description
High Performance Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) 

Reference Range

Nicotinamide
Sex Age From Age To Type Range Range Unit
       88-400 nmol/L 
Nicotinic Acid
Sex Age From Age To Type Range Range Unit
       Less than or equal to 80 nmol/L 
Nicotinuric Acid
Sex Age From Age To Type Range Range Unit
       Less than or equal to 55 nmol/L 

Special Info

Patient should fast for 4 hours and avoid the use of vitamin supplements for 24 hours prior to collection. Hemolyzed samples or samples collected in EDTA tubes are unacceptable.