Thymidine and Deoxyuridine Analytes (Plasma)
Test Mnemonic
PLTHY
CPT Codes
- 82542 - QTY (1)
Includes
- Thymidine
- Deoxyuridine
- Interpretation
Performing Laboratory
LabCorp
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Plasma | Sodium heparin (Green) | Frozen, ASAP | Separate plasma from cells ASAP and transfer to standard aliquot tube and freeze. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Plasma | EDTA (Lavender) | Frozen, ASAP | Separate plasma from cells ASAP and transfer to standard aliquot tube and freeze. | |
| 1 mL | Plasma | ACD A or B (Yellow) | Frozen, ASAP | Separate plasma from cells ASAP and transfer to standard aliquot tube and freeze. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.5 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | After separation from cells: Unacceptable |
| Refrigerated | After separation from cells: 24 hours |
| Frozen | After separation from cells: Indefinitely |
Days Performed
Varies
Turnaround Time
11 - 15 days
Methodology
| Name | Description |
|---|---|
| Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) |
Reference Range
Special Info
Thawed samples will be rejected.
Clinical Info
Plasma Thymidine/Deoxyuridine analyte is used for diagnosis of Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE). MNGIE is an autosomal recessive disorder caused by mutations in the gene encoding thymidine phosphorylase (TP). The disease is characterized clinically by impaired eye movements, gastrointestinal dysmotility, cachexia, peripheral neuropathy, myopathy and leukoencephalopathy. Molecular genetic studies of MNGIE patients\tissues have revealed multiple deletions, depletion, and site-specific point mutations of mitochrondrial DNA. TP is a cytosolic enzyme required for nucleoside homeostatis. In MNGIE, TP activity is severely reduced and consequently levels of thymidine and deoxyuridine in plasma are dramatically elevated. MNGIE patients may benefit from hematopoietic stem cell transplantation.