Immunoglobulin Kappa Chain using Biomed-2 PCR Primers Other
Test Mnemonic
IGKO
CPT Codes
- 81264 - QTY (1)
- G0452 - QTY (1)
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| One | Paraffin block, formalin fixed | Formalin-fixed, paraffin-embedded block | N/A | N/A | Paraffin-embedded tissue should be delivered to Surgical Pathology for accessioning and cutting. Specimens that undergo heavy metal fixation or are decalcified with strong acids (i.e., formic acid) are not acceptable for molecular testing. Non-decalcified specimens or samples that undergo EDTA decalcification are acceptable. Cut 10 charged, unbaked slides, 7um thick, include Pre H&E slide. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 6 ug | Extracted DNA | Clean container | Ambient | Ambient | Please indicate the source tissue for the DNA. |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Frozen tissue- unacceptable; Paraffin-embedded tissue- indefinitely; Extracted DNA- 24 hours |
| Refrigerated | Frozen tissue- unacceptable; Paraffin-embedded tissue- unacceptable; Extracted DNA- 3 years |
| Frozen | Frozen tissue- indefinitely; Paraffin-embedded tissue- unacceptable; Extracted DNA unacceptable |
Days Performed
2 days per week
Turnaround Time
7 days
Methodology
| Name | Description |
|---|---|
| Capillary Electrophoresis (CE) | |
| Polymerase Chain Reaction (PCR) |