Acute Leukemia NGS Panel, Other




Test Mnemonic

HDONGS

CPT Codes

  • 81455 - QTY (1)

Aliases

  • CEBPA
  • HDONGS
  • Hematologic Neoplasm Dual NGS, Other
  • NPM1

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
TwentySlide(s), unstained & unbakedSlides, unstainedAmbientAmbientNeed 20 charged, unbaked, unstained slides or 10 x 7 micron curls (scrolls) plus 1 H&E slide with best tumor area circled containing at least 8% tumor

Stability

Environmental Condition Description
AmbientFFPE tissue and clot section slides and curls are transported and stored at ambient temperature or refrigerated indefinitely
RefrigeratedFFPE tissue and clot section slides and curls are transported and stored at ambient temperature or refrigerated indefinitely
FrozenUNACCEPTABLE

Days Performed

Mon - Fri

Turnaround Time

10 days

Methodology

Name Description
Next Gen Sequencing 

Special Info

RNA_genes: ABL1, ABL2, AFDN (MLLT4), ALK, BCL11B, BCL2, BCL3, BCL6, BCR, BIRC3, BLNK, CBFB, CBL, CCND1, CCND2, CCND3, CD274, CD28, CDK6, CDKN2A, CEBPA, CEBPD, CEBPE, CEBPG, CHD1, CHIC2, CIITA, CREBBP, CRLF2, CSF1R, CTLA4, DEK, DGKH, DUSP22, EBF1, EIF4A1, EPOR, ERG, ETV6, FGFR1, FLT3, FOXP1, GLIS2, HLF, ID4, IKZF1, IKZF2, IKZF3, IL2RB, IRF4, IRF8, ITK, JAK2, KAT6A, KLF2, KMT2A, LMO1, LMO2, LYN, MALT1, MECOM, MEF2D, MLF1, MLLT10, MRTFA (MKL1), MUC1, MYC, MYH11, NF1, NFKB2, NOTCH1, NTRK3, NUP214, NUP98, NUTM1, P2RY8, PAG1, PAX5, PBX1, PDCD1, PDCD1LG2, PDGFRA, PDGFRB, PICALM, PML, PRDM16, PTK2B, RARA, RBM15, ROS1, RUNX1, RUNX1T1, SEMA6A, SETD2, STIL, SYK, TAL1, TCF3, TFG, TLX1, TLX3, TP63, TSLP, TYK2, VAV1, ZCCHC7, ZNF384. DNA_genes: ABL1, ASXL1, BCOR, BCORL1, BRAF, CALR, CBL, CDKN2A, CEBPA, CSF3R, CUX1, DDX41, DNMT3A, EED, ETNK1, ETV6, EZH2, FBXW7, FLT3, GATA1, GATA2, GNAS, IDH1, IDH2, IKZF1, JAK2, JAK3, KDM6A, KIT, KMT2A, KRAS, LUC7L2, MPL, MYD88, NF1, NOTCH1, NPM1, NRAS, PAX5, PHF6, PIGA, PPM1D, PRPF8, PTEN, PTPN11, RAD21, RIT1, RUNX1, SETBP1, SF3B1, SH2B3, SMC1A, SMC3, SRSF2, STAG2, STAT3, STAT5B, SUZ12, TET2, TP53, U2AF1, WT1, ZRSR2.

Clinical Info

DNA and RNA combination testing to evaluate for single nucleotide variants, small insertions and deletions, and common fusions in acute hematologic malignancies (acute myeloid and acute lymphoblastic leukemias); content includes all of the fusion targets of Hematologic Neoplasm Fusion NGS Panel as well as all of the DNA targets in the Chronic Myeloid NGS Panel.

Clinical Limitation

Testing of peripheral blood or fresh/buffy coat bone marrow aspirate is preferred to this test on FFPE when those samples are available and representative of disease state. Not to be ordered concurrently with the Hematologic Neoplasm Fusion NGS Panel or the Chronic Myeloid NGS Panel, since this panel includes all targeted regions from those panels.