Bacterial Culture and Gram Stain, Sterile Body Fluid




Test Mnemonic

BFCUL

CPT Codes

  • 87070 - QTY (1)
  • 87205 - QTY (1)
  • 87015 - QTY (1)
  • 87015 - QTY (1)
  • 87075 - QTY (1)
  • 87077 - QTY (1)
  • 87076 - QTY (1)
  • 87106 - QTY (1)
  • 87153 - QTY (1)
  • 87181 - QTY (1)
  • 87184 - QTY (1)
  • 87185 - QTY (1)
  • 87186 - QTY (1)
  • 87186 - QTY (1)
  • 87186 - QTY (1)
  • 87186 - QTY (1)

LOINC ®

611-4

Includes

  • Identification and susceptibility performed on postive cultures.

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
5 mLFluid, peritonealSterile containerAmbientRefrigerated 
5 mLFluid, pericardialSterile containerAmbientRefrigerated 
5 mLFluid, amnioticSterile containerAmbientRefrigerated 
5 mLFluid, pleuralSterile containerAmbientRefrigerated 
5 mLFluid, synovialSterile containerAmbientRefrigerated 

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 mL     

Stability

Environmental Condition Description
Ambient24 hours
FrozenUnacceptable
Refrigerated48 hours

Days Performed

Sun - Sat

Turnaround Time

7 days

Methodology

Name Description
Culture, Identification 
Stain 

Reference Range

Special Info

INVASIVELY COLLECTED synovial, peritoneal, pericardial, pleural, and amniotic fluids are acceptable for bacterial sterile body fluid culture. Specimens from indwelling DRAINS should be tested as "abscess and wound" culture and not as "sterile body fluid" culture. To collect the sterile body fluid, disinfect overlying skin with iodine or chlorhexidine preparation and obtain specimen with needle and syringe. Submit the fluid in a sterile specimen container or a capped syringe WITHOUT a needle. Swabs will be rejected. Fluid may be inoculated into a set of blood culture bottles (up to 10 ml per bottle) to maximize test sensitivity. If using bottles, also provide a separate aliquot of at least 1 ml for preparation of Gram stain and inoculation of solid media.

Clinical Info

If adequate volume of specimen is received, then normally sterile body fluids are cultured for aerobic and anaerobic bacteria using both solid and liquid nutrient media to provide opportunity for high specificity (solid media) and high sensitivity (broth media) culture.