Bile Acids, Total
Test Mnemonic
BILETO
CPT Codes
- 82239 - QTY (1)
Aliases
- Biliary Acids, Total
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Serum | SST (Gold) | Refrigerated | Allow specimen to clot completely at room temperature. Centrifuge within 2 hours of collection and refrigerate. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Serum | Red Plain | Refrigerated | Allow specimen to clot completely at room temperature. Centrifuge and transfer the serum to a plastic CCL tube within 2 hours of collection and refrigerate. | |
| 1 mL | Plasma | Lithium heparin (Green) | Refrigerated | Centrifuge and transfer the plasma to a plastic CCL tube within 2 hours of collection and refrigerate. | |
| 1 mL | Plasma | Lithium heparin PST (Lt. Green) | Refrigerated | Centrifuge within 2 hours of collection and refrigerate. | |
| 1 mL | Plasma | EDTA (Lavender) | Refrigerated | Centrifuge and transfer the plasma to a plastic CCL tube within 2 hours of collection and refrigerate. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.5 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 48 hours |
| Refrigerated | 30 days |
| Frozen | 30 days |
Days Performed
Mon - Sat
Turnaround Time
1 - 3 days
Methodology
| Name | Description |
|---|---|
| Enzymatic |
Special Info
The reference intervals for adults >20 years old were obtained using fasting blood specimens. Reference intervals for children, adults <20 years old and pregnant women were obtained using random blood draw specimens.
Clinical Info
Use for the in vitro quantitative determination of total bile acids in serum or plasma.
Clinical Limitation
Total bile acid concentration in serum is increased after meals; hence, samples should be collected under fasting conditions. This does not apply to women for whom intrahepatic cholestasis of pregnancy as a diagnostic consideration; these patients will need peak bile acid testing, and samples should be postprandial. This serum bile acid assay should not be used for patients receiving ursodeoxycholic acid, nor should it be used as the primary assay for the screening or diagnosis of genetic disorders of bile acid synthesis.