EGFR Hotspot PCR Analysis Assay
Test Mnemonic
EGFRID
CPT Codes
- 81235 - QTY (1)
Aliases
- EGFR
- EGFRGN
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
---|---|---|---|---|---|
10 mm square | Block, Formalin fixed paraffin | Formalin-fixed, paraffin-embedded block | Ambient | Ambient | Formalin-fixed paraffin-embedded tissue slides. Transport and store slides at ambient temperature. Three unstained sections FFPE tissue on charged, unbaked slides or scrolls, plus one H&E stained section with best tumor area circled by a pathologist. Provide the percentage of tumor cells present, minimum 10%. |
10 mm square | Cell block, formalin fixed paraffin | Formalin-fixed, paraffin-embedded block | Ambient | Ambient | Formalin-fixed paraffin-embedded cell block slides. Transport and store slides at ambient temperature. Three unstained sections FFPE cell block tissue on charged, unbaked slides. Provide the percentage of tumor cells present, minimum 10%. |
10 mm square | Aspirate, fine needle | Slide | Ambient | Ambient | One alcohol fixed cytology slide. Tissue adequacy must be assessed by cytopathologist. |
Stability
Environmental Condition | Description |
---|---|
Ambient | FFPET slides and scrolls, cell block slides and alcohol fixed slides are stable at ambient temperature indefinitely. |
Refrigerated | FFPET slides and scrolls, cell block slides and alcohol fixed slides are stable at ambient temperature indefinitely. |
Frozen | Unacceptable |
Days Performed
Mon - Fri
Turnaround Time
3 days
Methodology
Name | Description |
---|---|
Real-Time Polymerase Chain Reaction (RT-PCR) |
Special Info
Detects 51 known mutations of the EGFR gene will be tested.
Clinical Limitation
Detects 51 known mutations of the EGFR gene