FISH for DDIT3/GLI1(12q13.3)

Test Mnemonic


CPT Codes

  • 88271 - QTY (2)
  • 88275 - QTY (1)
  • 88291 - QTY (1)


  • GLI1
  • MLS
  • Myxoid Liposarcoma
  • DDIT3/GLI1

Performing Laboratory

Cleveland Clinic Laboratories

Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 slidesSlide (s), unstained AmbientAmbientOne formalin-fixed, paraffin embedded block containing representative tissue OR unstained 4 μm tissue sections on electrostatically charged slides containing representative tumor. Tissue must be fixed in formalin NOT B5 or PREFER fixative


Environmental Condition Description
AmbientBlocks/Slides stable at ambient temperatures.

Days Performed

5 days per week

Turnaround Time

5 - 7 days


Name Description
Fluorescent In-Situ Hybridization (FISH) 

Clinical Info

Interphase FISH analysis utilizing a dual color, break-apart probe for the DDIT3/GLI1 genes at 12q13.3. Rearrangements involving the DDIT3 (CHOP) gene are observed in myxoid/round cell liposarcoma. The translocation partner gene cannot be identified in this assay. This assay also detects GLI1 amplification, which may be seen in mesenchymal soft tissue neoplasms. If no cells are available for analysis, no analysis charges will be incurred.