FISH for ROS1 (6q22)




Test Mnemonic

ROS1

CPT Codes

  • 88377 - QTY (1)

Aliases

  • ROS1 gene rearrangement
  • ROS1 gene rearrangement by FISH

Includes

  • LSI 6q22.1

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 slidesParaffin block, formalin fixedSlidesAmbientAmbientPre and post H&E with two unstained slides

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 slides    Pre and post H&E with two unstained slides

Stability

Environmental Condition Description
AmbientSlides from FFPE Tissue at room temp

Days Performed

5 days per week

Turnaround Time

5 - 7 days

Methodology

Name Description
Fluorescent In-Situ Hybridization (FISH) 

Reference Range

Special Info

Pre and post H&E with two unstained slides

Clinical Info

Interphase FISH analysis utilizing a dual color, break-apart probe for the ROS1 gene at 6q22.1. Rearrangements involving ROS1 may be seen in individuals with late-stage, lung adenocarcinomas that are negative for EGFR and ALK rearrangements. The translocation partner gene cannot be identified with this assay. If no cells are available for analysis, no analysis charges will be incurred.

Clinical Reference

Shaw AT, Sai-Hong IOU, Yung-Jue B, et al. Crizotinib in ROS1-Rearranged Non–Small-Cell Lung. National Comprehensive Cancer Network (NCCN) Clinical Practice Guidelines in Oncology, Non-Small Cell Lun