Non-variola orthopoxvirus (includes Mpox or monkeypox virus)
Test Mnemonic
OPXPCR
Primary CPT Codes
- 87593 - QTY (1), Amplification, Orthopox virus
LOINC ®
104610-1
Aliases
- Vaccinia virus
- Non-variola orthopoxvirus
- Monkeypox Virus Qualitative PCR
- Monkey
- Monkeypox
- Mpox
Includes
- Orthopoxvirus Source
- Orthopoxvirus by PCR
Performing Laboratory
Cleveland Clinic Laboratories
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| Swab | Fluid, lesion | Viral Transport Media | Refrigerated | Using a sterile swab, apply firm pressure to the lesion and swipe back and forth at least 2-3 times before rotating the swab and repeating using the other side of the swab. If the lesion ruptures while swabbing, ensure to collect the lesion fluid. Specimen source required. |
Stability
| Environmental Condition | Description |
|---|---|
| Refrigerated | 7 days |
| Ambient | 48 hours |
| Frozen | Unacceptable |
Days Performed
Varies
Turnaround Time
Same day
Methodology
| Name | Description |
|---|---|
| Qualitative Polymerase Chain Reaction |
Reference Range
| Monkeypox virus Clade II DNA | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | Not Detected |
Special Info
Specimen source required. Calcium alginate swabs, wooden swabs and specimens without swabs will be rejected.
Clinical Info
This qualitative PCR test is used to detect both monkeypox virus clade II and non-variola Orthopoxvirus DNA. Detection of non-variola Orthopoxvirus DNA only most likely represents the presence of monkeypox virus clade II, however, there is a small chance this could represent the presence of a different non-variola Orthopoxvirus. Correlation with patient history is advised.
Clinical Limitation
This test is intended for the detection of both monkeypox virus clade II and non-variola Orthopoxvirus DNA. A negative result does not rule out the presence of PCR inhibitors in the patient specimen or assay specific nucleic acid in concentrations below the level of detection by the assay. Clinical correlation with patient history and other diagnostic information is necessary to determine patient infection status.