PML :: RARA Detection by RT-PCR, Quantitative
Test Mnemonic
APLPCR
CPT Codes
- 81315 - QTY (1)
Aliases
- AMPL' APL
- APL MRD
- APL Post-consolidation therapy testing
- PML-RARA fusion types, A, B, B variant
- PML-RARA long, short, variable types
- PML-RARA S, L, V forms
- PML::RARA fusion types
- PML::RARA fusion types, A, B, B variant
- PML::RARA long, short, variable types
- PML::RARA S, L, V forms
- PML_RARA fusion types
- Quantitative PML
- t(15;17)
Includes
- PML::RARA Translocation, Source
- PML::RARA Translocation, Result
- PML::RARA Translocation, NCN
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 5 mL | Whole blood | EDTA (Lavender) | Refrigerated | Critical refrigerated. Specimen must be delivered to Cleveland Clinic Laboratories by 3 p.m. EST on the day of collection. DO NOT collect the day before or the day of a major holiday. Separate specimens must be submitted when multiple tests are ordered. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 3 mL | Bone marrow | EDTA (Lavender) | Refrigerated | Critical refrigerated. Specimen must be delivered to Cleveland Clinic Laboratories by 3 p.m. EST on the day of collection. DO NOT collect the day before or the day of a major holiday. Separate specimens must be submitted when multiple tests are ordered. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Bone marrow | ||||
| 3 mL | Whole blood |
Stability
| Environmental Condition | Description |
|---|---|
| Refrigerated | 48 hours |
| Frozen | Unacceptable |
| Ambient | Unacceptable |
Days Performed
Varies
Turnaround Time
3 - 10 days
Methodology
| Name | Description |
|---|---|
| Reverse Transcription/Polymerase Chain Reaction (RT/PCR) |
Reference Range
Special Info
CRITICAL REFRIGERATED. Severely hemolyzed or clotted specimens will be rejected.
Clinical Info
Use to detect and quantitate PML::RARA fusion transcripts in individuals with acute promyelocytic leukemia. Use to monitor minimal residual disease and assess the risk of disease relapse.
Clinical Limitation
Testing is not New York state approved. This test detects fusion types 1, 2, and 3; it does NOT identify which transcript is detected.