Urine Free Cortisol by LC-MS/MS




Test Mnemonic

UFRCRT

CPT Codes

  • 82530 - QTY (1)
  • 82530 - QTY (1)

Includes

  • Free Cortisol (ug/L)
  • Creatinine (mg/dL)
  • Free Cortisol (ug/g crt)
  • Free Cortisol (ug/24 hour)
  • Creatinine (mg/24 hour)

Performing Laboratory

ARUP


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
4 mLUrine, 24-hour (well-mixed)24 HR Urine ContainerRefrigerate during collection.Refrigerated 

Alternate Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
4 mLUrine, randomClean container Refrigerated 

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mL     

Stability

Environmental Condition Description
AmbientUnacceptable
Refrigerated2 weeks
Frozen6 months

Days Performed

Sun - Sat

Turnaround Time

2 - 6 days

Methodology

Name Description
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) 

Reference Range

Free Cortisol
Sex Age From Age To Type Range Range Unit
 3 Years8 YearsNormal< or = 18ug/24 hrs
 9 Years12 YearsNormal< or = 37ug/24 hrs
 13 Years17 YearsNormal< or = 56ug/24 hrs
Female18 Years99 YearsNormal< or = 45ug/24 hrs
Male18 Years99 YearsNormal< or = 60ug/24 hrs
Free Urine Cortisol
Sex Age From Age To Type Range Range Unit
Female      NormalPregnancy: <59ug/g crt
Female      NormalPrepubertal: <25ug/g crt
Female18 Years99 YearsNormal< 24ug/g crt
Male      NormalPrepubertal: <25ug/g crt
Male18 Years99 YearsNormal< 32ug/g crt

Special Info

Indicate period and volume on requisition. Acidified specimens and specimens with preservatives are unacceptable. This test is New York DOH approved.

Clinical Info

The optimal specimen for this testing is a 24-hour urine collection. Mass per day calculations are not reported for the following specimen types: a random collection, a collection with duration of less than 20 hours, a collection with duration greater than 28 hours, or a collection with total volume less than 400 mL or greater than 5000 mL. Ratios to creatinine may be useful for these evaluations. Baseline urinary free cortisol excretions less than 5 ug/day may be consistent with adrenal insufficiency.