Urine Free Cortisol by LC-MS/MS




Test Mnemonic

UFRCRT

CPT Codes

  • 82530 - QTY (1)
  • 82530 - QTY (1)

Includes

  • Free Cortisol (ug/L)
  • Creatinine (mg/dL)
  • Free Cortisol (ug/g crt)
  • Free Cortisol (ug/24 hour)
  • Creatinine (mg/24 hour)

Performing Laboratory

ARUP


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
4 mLUrine, 24-hour (well-mixed)Clean containerRefrigerate during collection.Refrigerated 

Alternate Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
4 mLUrine, randomClean container Refrigerated 

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mL     

Stability

Environmental Condition Description
AmbientUnacceptable
Refrigerated2 weeks
Frozen6 months

Days Performed

Sun - Sat

Turnaround Time

2 - 5 days

Methodology

Name Description
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) 

Reference Range

Free Cortisol
Sex Age From Age To Type Range Range Unit
 3 Years8 YearsNormal< or = 18ug/24 hrs
 9 Years12 YearsNormal< or = 37ug/24 hrs
 13 Years17 YearsNormal< or = 56ug/24 hrs
Female18 Years99 YearsNormal< or = 45ug/24 hrs
Male18 Years99 YearsNormal< or = 60ug/24 hrs
Free Urine Cortisol
Sex Age From Age To Type Range Range Unit
Female      NormalPregnancy: <59ug/g crt
Female      NormalPrepubertal: <25ug/g crt
Female18 Years99 YearsNormal< 24ug/g crt
Male      NormalPrepubertal: <25ug/g crt
Male18 Years99 YearsNormal< 32ug/g crt

Special Info

Indicate period and volume on requisition. Acidified specimens and specimens with preservatives are unacceptable.

Clinical Info

The optimal specimen for this testing is a 24-hour urine collection. Mass per day calculations are not reported for the following specimen types: a random collection, a collection with duration of less than 20 hours, a collection with duration greater than 28 hours, or a collection with total volume less than 400 mL or greater than 5000 mL. Ratios to creatinine may be useful for these evaluations. Baseline urinary free cortisol excretions less than 5 ug/day may be consistent with adrenal insufficiency.