Vitamin D, 1,25-Dihydroxy
Test Mnemonic
125VTD
CPT Codes
- 82652 - QTY (1)
Aliases
- 1,25 Dihydroxyvitamin D
- Calcitriol
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.75 mL | Serum | SST (Gold) | Refrigerated |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.75 mL | Plasma | Lithium heparin PST (Lt. Green) | |||
| 0.75 mL | Plasma | EDTA (Lavender) | Plasma samples should be centrifuged and the plasma removed from the cells immediately after centrifugation. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.4 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 7 days |
| Refrigerated | 14 days |
| Frozen | 30 days |
Days Performed
Mon - Fri
Turnaround Time
1 - 4 days
Methodology
| Name | Description |
|---|---|
| Chemiluminescence Immunoassay (CLIA) |
Reference Range
| Vitamin D, 1,25-Dihydroxy | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 19.9-79.3 | pg/mL |
Special Info
Samples may be frozen-thawed up to 4 times.
Clinical Info
Aid in the diagnosis of primary hyperparathyroidism, hypoparathyroidism, pseudohypoparathyroidism, renal osteodystrophy, vitamin D resistant rickets.
Clinical Limitation
Grossly hemolyzed samples, lipemic samples, and samples containing particulate matter or exhibiting obvious microbial contamination will be rejected. Do not heat inactivate serum or plasma. Heterophilic antibodies in human serum can react with reagent immunoglobulins, interfering with the assay. Patients routinely exposed to animals or to animal serum products can be prone to this interference and anomalous values may be observed. The assay has been shown to cross-react with Zemplar (paricalcitol) an active form of Vitamin D.