Pneumococcal IgG Antibodies, 14 Serotypes
Test Mnemonic
PNEUMG
CPT Codes
- 86317 - QTY (14)
Aliases
- Streptococcus pneumoniae Antibodies
Includes
- Streptococcus pneumoniae Serotype 1 Abs
- Streptococcus pneumoniae Serotype 3 Abs
- Streptococcus pneumoniae Serotype 4 Abs
- Streptococcus pneumoniae Serotype 5 Abs
- Streptococcus pneumoniae Serotype 6B Abs
- Streptococcus pneumoniae Serotype 7F Abs
- Streptococcus pneumoniae Serotype 8 Abs
- Streptococcus pneumoniae Serotype 9N Abs
- Streptococcus pneumoniae Serotype 9V Abs
- Streptococcus pneumoniae Serotype 12F Abs
- Streptococcus pneumoniae Serotype 14 Abs
- Streptococcus pneumoniae Serotype 18C Abs
- Streptococcus pneumoniae Serotype 19F Abs
- Streptococcus pneumoniae Serotype 23F Abs
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1.5 mL | Serum | SST (Gold) | Refrigerated | Post-immunization specimen should be drawn 30 days after immunization and must be received within 60 days of pre-immunization specimen. Label specimens clearly as 'Pre' or 'Post.' Separate serum from cells ASAP or within 2 hours of collection and transfer into standard aliquot tube. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.25 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | After separation from cells: 48 hours |
| Frozen | After separation from cells: 60 days (Avoid repeated freeze/thaw cycles) |
| Refrigerated | After separation from cells: 2 weeks |
Days Performed
Sun - Sat
Turnaround Time
2 - 4 days
Methodology
| Name | Description |
|---|---|
| Quantitative Multiplex Chemiluminescent Immunoassay |
Reference Range
Special Info
Post-immunization specimen should be drawn 30 days after immunization and, if shipped separately, must be received within 60 days of pre-immunization specimen. Pre-immunization samples will be held and tested simultaneously with post-immunization samples. Plasma or other body fluids will be rejected. Contaminated, hemolyzed, or severely lipemic specimens are unacceptable. This test is New York DOH approved.
Clinical Info
A pre- and post-vaccination comparison is required to adequately assess the humoral immune response to Prevnar 7 (P7), Prevnar 13 (P13), and/or Pneumovax 23 (PNX) Streptococcus pneumoniae vaccines. Pre-vaccination samples should be collected prior to vaccine administration. Post-vaccination samples should be obtained at least 4 weeks after immunization. Testing of post-vaccination samples alone will provide only general immune status of the individual to various pneumococcal serotypes. In the case of pure polysaccharide vaccine, indication of immune system competence is further delineated as an adequate response to at least 50 percent of the serotypes in the vaccine challenge for those 2-5 years of age and to at least 70 percent of the serotypes in the vaccine challenge for those 6-65 years of age. Individual immune response may vary based on age, past exposure, immunocompetence, and pneumococcal serotype. Responder Status Antibody Ratio Non-Responder . . . . . . . . . . . . . . Less than 2-fold Weak Responder . . . . . . . . . . . . . 2-fold to 4-fold Good Responder . . . . . . . . . . . . . Greater than 4-fold A response to 50-70 percent or more of the serotypes in the vaccine challenge is considered a normal humoral response1. Antibody concentration greater than 1.0 – 1.3 µg/mL is generally considered long-term protection2. References: 1. Daly TM, Pickering JW, Zhang X, Prince HE, Hill HR. Multilaboratory assessment of threshold versus fold-change algorithms for minimizing analytical variability in multiplexed pneumococcal IgG measurements. Clin Vaccine Immunol. 2014;21(7):982-8. 2. Daly TM, Hill HR. Use and Clinical Interpretation of Pneumococcal Antibody Measurements in the Evaluation of Humoral Immune Function. Clin Vaccine Immunol. 2015;22(2):148-152.