FISH for Cutaneous Melanoma
Test Mnemonic
CMFISH
CPT Codes
- 88377 - QTY (2)
Includes
- FISH for 6p24.1, 6q23.3, 11q13.3, CEP 6
- FISH for 9p21.3, 8q24.21, CEP 9
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| Three | Slide (s), unstained | Clean container | One formalin-fixed, paraffin embedded block containing representative tissue OR unstained 4 μm tissue sections on electrostatically charged slides containing representative tumor. Tissue must be fixed in formalin NOT B5 or PREFER fixative |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | FFPE tissue block or unstained slides from FFPE tissue are stable in ambient environment. |
Days Performed
5 days per week
Turnaround Time
6 - 10 days
Methodology
| Name | Description |
|---|---|
| Fluorescent In-Situ Hybridization (FISH) |
Clinical Info
Two interphase FISH assays detecting copy number variations of the RREB1 (6p23.3), MYB (6q23.3), CCND1 (11q13.3), and CDKN2A (9p21.3) genes, as well as MYC (8q24.21) amplification. Useful in diagnosing and classifying cutaneous melanoma. If no cells are available for analysis, no analysis charges will be incurred.