ACTH Stimulation, 3 Time Points
Test Mnemonic
ACTHST
CPT Codes
- 80400 - QTY (1)
- 82533 - QTY (1)
Aliases
- Cortisol Stimulation 3 Time Points
- Cortisol Stimulation 3 Time Points
- Cortisol Stimulation 3 Time Points
- Cortrosyn Stimulation
- Cortrosyn Stimulation
- Cortrosyn Stimulation
Includes
- Cortisol Basal
- Cortisol 30 minutes
- Cortisol 60 minutes
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Plasma | Lithium heparin PST (Lt. Green) | Refrigerated | Collect a baseline specimen, then collect specimens at 30 minutes and 60 minutes after intravenous/intramuscular administration of cortrosyn (cosyntropin) |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Serum | SST (Gold) | Refrigerated | Collect a baseline specimen, then collect specimens at 30 minutes and 60 minutes after intravenous/intramuscular administration of cortrosyn (cosyntropin) | |
| 1 mL | Plasma | EDTA (Lavender) | Centrifuge, aliquot and refrigerate. | Collect a baseline specimen, then collect specimens at 30 minutes and 60 minutes after intravenous/intramuscular administration of cortrosyn (cosyntropin) |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.3 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 24 hours |
| Refrigerated | 4 days |
| Frozen | 12 months |
Days Performed
Sun - Sat
Turnaround Time
24 hours
Methodology
| Name | Description |
|---|---|
| Electro Chemiluminescence Immunoassay (ECLIA) |
Reference Range
| Cortisol, Basal | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | Morning hours 6:00 to 10:00 am: 4.8-19.5 | ug/dL | Normal | Afternoon hours 4:00 to 8:00 pm: 2.5-11.9 | ug/dL |
Special Info
Patients taking a biotin dose of up to 5 mg/day should refrain from taking biotin for 4 hours prior to sample collection. Patients taking a biotin dose of 5 to 10 mg/day should refrain from taking biotin for 8 hours prior to sample collection. Patients taking a biotin dose > 10 mg/day should consult with their physician or the laboratory prior to having a sample taken. Clinicians should consider biotin interference as a source of error, when clinically suspicious of the laboratory result.
Clinical Info
Diagnosis of adrenal insufficiency.
Clinical Limitation
During metyrapon tests, 11 deoxycortisol levels are elevated. Falsely elevated cortisol values may be determined due to cross reactions. In samples from patients who have been treated with prednisolone, 6-alpha-Methylprednisolone or prednisone, falsely elevated concentrations of cortisol may be determined.