Acute Leukemia NGS Panel, Other
Test Mnemonic
HDONGS
CPT Codes
- 81455 - QTY (1)
Aliases
- CEBPA
- HDONGS
- Hematologic Neoplasm Dual NGS, Other
- NPM1
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| Twenty | Slide(s), unstained & unbaked | Slides, unstained | Ambient | Ambient | Need 20 charged, unbaked, unstained slides or 10 x 7 micron curls (scrolls) plus 1 H&E slide with best tumor area circled containing at least 8% tumor |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | FFPE tissue and clot section slides and curls are transported and stored at ambient temperature or refrigerated indefinitely |
| Refrigerated | FFPE tissue and clot section slides and curls are transported and stored at ambient temperature or refrigerated indefinitely |
| Frozen | UNACCEPTABLE |
Days Performed
Mon - Fri
Turnaround Time
10 days
Methodology
| Name | Description |
|---|---|
| Next Gen Sequencing |
Special Info
RNA_genes: ABL1, ABL2, AFDN (MLLT4), ALK, BCL11B, BCL2, BCL3, BCL6, BCR, BIRC3, BLNK, CBFB, CBL, CCND1, CCND2, CCND3, CD274, CD28, CDK6, CDKN2A, CEBPA, CEBPD, CEBPE, CEBPG, CHD1, CHIC2, CIITA, CREBBP, CRLF2, CSF1R, CTLA4, DEK, DGKH, DUSP22, EBF1, EIF4A1, EPOR, ERG, ETV6, FGFR1, FLT3, FOXP1, GLIS2, HLF, ID4, IKZF1, IKZF2, IKZF3, IL2RB, IRF4, IRF8, ITK, JAK2, KAT6A, KLF2, KMT2A, LMO1, LMO2, LYN, MALT1, MECOM, MEF2D, MLF1, MLLT10, MRTFA (MKL1), MUC1, MYC, MYH11, NF1, NFKB2, NOTCH1, NTRK3, NUP214, NUP98, NUTM1, P2RY8, PAG1, PAX5, PBX1, PDCD1, PDCD1LG2, PDGFRA, PDGFRB, PICALM, PML, PRDM16, PTK2B, RARA, RBM15, ROS1, RUNX1, RUNX1T1, SEMA6A, SETD2, STIL, SYK, TAL1, TCF3, TFG, TLX1, TLX3, TP63, TSLP, TYK2, VAV1, ZCCHC7, ZNF384. DNA_genes: ABL1, ASXL1, BCOR, BCORL1, BRAF, CALR, CBL, CDKN2A, CEBPA, CSF3R, CUX1, DDX41, DNMT3A, EED, ETNK1, ETV6, EZH2, FBXW7, FLT3, GATA1, GATA2, GNAS, IDH1, IDH2, IKZF1, JAK2, JAK3, KDM6A, KIT, KMT2A, KRAS, LUC7L2, MPL, MYD88, NF1, NOTCH1, NPM1, NRAS, PAX5, PHF6, PIGA, PPM1D, PRPF8, PTEN, PTPN11, RAD21, RIT1, RUNX1, SETBP1, SF3B1, SH2B3, SMC1A, SMC3, SRSF2, STAG2, STAT3, STAT5B, SUZ12, TET2, TP53, U2AF1, WT1, ZRSR2.
Clinical Info
DNA and RNA combination testing to evaluate for single nucleotide variants, small insertions and deletions, and common fusions in acute hematologic malignancies (acute myeloid and acute lymphoblastic leukemias); content includes all of the fusion targets of Hematologic Neoplasm Fusion NGS Panel as well as all of the DNA targets in the Chronic Myeloid NGS Panel.
Clinical Limitation
Testing of peripheral blood or fresh/buffy coat bone marrow aspirate is preferred to this test on FFPE when those samples are available and representative of disease state. Not to be ordered concurrently with the Hematologic Neoplasm Fusion NGS Panel or the Chronic Myeloid NGS Panel, since this panel includes all targeted regions from those panels.