Alkaline Phosphatase Isoenzymes




Test Mnemonic

ALKISO

CPT Codes

  • 84075 - QTY (1)
  • 84080 - QTY (1)

Aliases

  • Alkaline Phosphatase Fractionation

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 mLSerumSST (Gold)N/ARefrigerated 

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1.5 mL     

Stability

Environmental Condition Description
Ambient24 hours
Refrigerated7 days
Frozen14 days

Days Performed

Varies, Mon - Fri

Turnaround Time

3 - 6 days

Methodology

Name Description
Electrophoresis 

Reference Range

Alkaline Phosphatase
Sex Age From Age To Type Range Range Unit
   Days14 DaysNormal83 - 248U/L
 15 Days364 DaysNormal122 - 469U/L
 1 Years9 YearsNormal142 - 335U/L
 10 Years12 YearsNormal129 - 417U/L
Male13 Years14 YearsNormal116 - 468U/L
Male15 Years16 YearsNormal82 - 331U/L
Male17 Years18 YearsNormal55 - 149U/L
Male19 Years99 YearsNormal38 - 113U/L
Female13 Years14 YearsNormal57 - 254U/L
Female15 Years16 YearsNormal50 - 117U/L
Female17 Years18 YearsNormal45 - 87U/L
Female19 Years99 YearsNormal34 - 123U/L
Bone %
Sex Age From Age To Type Range Range Unit
       Normal10.7-68.3%
Bone Fraction
Sex Age From Age To Type Range Range Unit
       Normal12.9-52.6U/L
Intestinal %
Sex Age From Age To Type Range Range Unit
       Normal0.0-24.2%
Intestine Fraction
Sex Age From Age To Type Range Range Unit
       Normal0.0-16.3U/L
Liver %
Sex Age From Age To Type Range Range Unit
       Normal26.0-86.2%
Liver Fraction
Sex Age From Age To Type Range Range Unit
       Normal16.0-69.3U/L

Special Info

Patient should be fasting. Patients who have B or O blood group and are secretors may have an elevated ALP about two hours after a fatty meal. Age and sex of patient are necessary for interpretation of results.

Clinical Info

When the Total Alkaline Phosphatase activity is increased, the Isoenzymes are useful in determining the source of the increased activity.

Clinical Limitation

High concentrations of phosphate, oxalate, citrate and cyanide will inhibit alkaline phosphatase (ALP) activity.Excess glycine may inhibit ALP activity by complexing Mg2+. EDTA inhibits some of the isoenzymes of ALP. Do not use as an anticoagulant. Several drugs cause an enzymatic imbalance which may change the ALP level.