B Cell CD20 Expression
Test Mnemonic
CD20
CPT Codes
- 86355 - QTY (1)
- 86356 - QTY (1)
Aliases
- CD20 on B Cells
- Rituximab Therapy CD20
Includes
- CD19+ Percent of Lymphocytes
- CD19+ Count of Lymphocytes
- CD20+ Percent of CD19+ Cells
- CD20+ Count of CD19+ Cells
- Cell Viability
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 5 mL | Whole blood | Sodium heparin (Green) | Ambient |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 5 mL | Whole blood | EDTA (Lavender) | Ambient |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 72 hours |
| Refrigerated | 72 hours |
| Frozen | Unacceptable |
Days Performed
Sun - Sat
Turnaround Time
2 - 3 days
Methodology
| Name | Description |
|---|---|
| Flow Cytometry (FC) |
Special Info
Provide clinical history, differential diagnosis, and any relevant pathology reports. Clotted, hemolyzed, or frozen specimens will be rejected. This test is New York State approved.
Clinical Info
Monoclonal antibody-based therapies, such as rituximab that target the CD20 antigen, are being used to treat patients with a variety of autoimmune disorders. The effectiveness of this therapy is dependent on the degree of B-cell suppression and varies by disease state. This assay is designed to detect low levels of B cells and provide quantitative cell numbers in the setting of rituximab-treated patients using both CD20 and CD19.