Bioavailable Testosterone/SHBG, Female & Child | Cleveland Clinic Laboratories

Bioavailable Testosterone/SHBG, Female & Child Print

Test Mnemonic

BTSTFC

CPT Codes

84403 - QTY (1)
84402 - QTY (1)
84270 - QTY (1)

Includes

Testosterone
Testosterone, Free
Sex Hormone Binding Globulin
Testosterone, Bioavailable by Mass Spec

Performing Laboratory

ARUP

Specimen Requirements

Volume	Type	Container	Collect Temperature	Transport Temperature	Special Instructions
1 mL	Serum	SST (Gold)		Refrigerated	Collection between 6 - 10 a.m. preferred but not required. Separate serum from cells ASAP or within 2 hours of collection. See Clinical Information for additional details.

Alternate Specimen Requirements

Volume	Type	Container	Collect Temperature	Transport Temperature	Special Instructions
1 mL	Plasma	Sodium or Lithium heparin (Green)		Refrigerated	Collection between 6 - 10 a.m. preferred but not required. Separate plasma from cells ASAP or within 2 hours of collection. See Clinical Information for additional details.

Minimum Specimen Requirements

Volume	Type	Container	Collect Temperature	Transport Temperature	Special Instructions
0.8 mL

Stability

Environmental Condition	Description
Ambient	After separation from cells: 24 hours
Refrigerated	After separation from cells: 1 week
Frozen	After separation from cells: 6 months

Days Performed

Sun - Sat

Turnaround Time

2 - 6 days

Methodology

Name	Description
Electro Chemiluminescence Immunoassay (ECLIA)
High Performance Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)
Calculation

Reference Range

Sex Hormone Binding Globulin
Sex	Age From	Age To	Type	Range	Range Unit
Female			Normal	Tanner Stage I: 30 - 173	nmol/L
Female			Normal	Tanner Stage II: 16 - 127	nmol/L
Female			Normal	Tanner Stage III: 12 - 98	nmol/L
Female			Normal	Tanner Stage IV: 14 - 151	nmol/L
Female			Normal	Tanner Stage V: 23 - 165	nmol/L
Female	1 Days	30 Days	Normal	14 - 60	nmol/L
Female	31 Days	364 Days	Normal	60 - 215	nmol/L
Female	1 Years	3 Years	Normal	60 - 190	nmol/L
Female	4 Years	6 Years	Normal	55 - 170	nmol/L
Female	7 Years	9 Years	Normal	35 - 170	nmol/L
Female	10 Years	12 Years	Normal	17 - 155	nmol/L
Female	13 Years	15 Years	Normal	11 - 120	nmol/L
Female	16 Years	17 Years	Normal	19 - 145	nmol/L
Female	18 Years	49 Years	Normal	25 - 122	nmol/L
Female	50 Years	99 Years	Normal	17 - 125	nmol/L
Male			Normal	Tanner Stage I: 26 - 186	nmol/L
Male			Normal	Tanner Stage II: 22 - 169	nmol/L
Male			Normal	Tanner Stage III: 13 - 104	nmol/L
Male			Normal	Tanner Stage IV: 11 - 60	nmol/L
Male			Normal	Tanner Stage V: 11 - 71	nmol/L
Male	1 Days	30 Days	Normal	13 - 85	nmol/L
Male	31 Days	364 Days	Normal	70 - 250	nmol/L
Male	1 Years	3 Years	Normal	50 - 180	nmol/L
Male	4 Years	6 Years	Normal	45 - 175	nmol/L
Male	7 Years	9 Years	Normal	28 - 190	nmol/L
Male	10 Years	12 Years	Normal	23 - 160	nmol/L
Male	13 Years	15 Years	Normal	13 - 140	nmol/L
Male	16 Years	17 Years	Normal	10 - 60	nmol/L
Male	18 Years	49 Years	Normal	17 - 56	nmol/L
Male	50 Years	99 Years	Normal	19 - 76	nmol/L
Testosterone
Sex	Age From	Age To	Type	Range	Range Unit
Female			Normal	Newborn: 20 - 64	ng/dL
Female	2 Years	3 Years	Normal	Less than 20	ng/dL
Female	6 Years	7 Years	Normal	Less than 7	ng/dL
Female	6 Months	24 Months	Normal	Less than 9	ng/dL
Female	4 Years	5 Years	Normal	Less than 30	ng/dL
Female			Normal	Postmenopausal: 5 - 32	ng/dL
Female			Normal	Premature (26 - 28 weeks): 5 - 16	ng/dL
Female			Normal	Premature (31 - 35 weeks): 5 - 22	ng/dL
Female			Normal	Premenopausal (Greater than 18 years): 9 - 55	ng/dL
Female			Normal	Tanner Stage I: 2 -17	ng/dL
Female			Normal	Tanner Stage II: 5 - 40	ng/dL
Female			Normal	Tanner Stage III: 10 - 63	ng/dL
Female			Normal	Tanner Stage IV - V: 11 - 62	ng/dL
Female	1 Months	5 Months	Normal	Less than 20	ng/dL
Female	8 Years	9 Years	Normal	1 - 11	ng/dL
Female	10 Years	11 Years	Normal	3 - 32	ng/dL
Female	12 Years	13 Years	Normal	6 - 50	ng/dL
Female	14 Years	15 Years	Normal	6 - 52	ng/dL
Female	16 Years	17 Years	Normal	9 - 58	ng/dL
Female	18 Years	39 Years	Normal	9 - 55	ng/dL
Female	40 Years	59 Years	Normal	9 - 55	ng/dL
Female	60 Years	99 Years	Normal	5 - 32	ng/dL
			Normal
			Normal	>/=1.00
			Normal	2.22
			Normal	<>6.00
			Normal	=/<33
			Normal	=/>66

				>/=10.00
				=/<7.50
			Urgent
			Urgent	<>3.00
			Urgent	>6 or <7
			Critical	>/<4.00
			Critical	<>3.00
			Critical	>/5.00
Male			Normal	Newborn: 75 - 400	ng/dL
Male			Normal	Premature (26 - 28 weeks): 59 - 125	ng/dL
Male			Normal	Premature (31- 35 weeks): 37 - 198	ng/dL
Male			Normal	Tanner Stage I: 2 -15	ng/dL
Male			Normal	Tanner Stage II: 3 - 303	ng/dL
Male			Normal	Tanner Stage III: 10 - 851	ng/dL
Male			Normal	Tanner Stage IV - V: 162 - 847	ng/dL
Male	1 Months	5 Months	Normal	14 - 363	ng/dL
Male	6 Months	24 Months	Normal	Less than 37	ng/dL
Male	2 Years	3 Years	Normal	Less than 15	ng/dL
Male	4 Years	5 Years	Normal	Less than 19	ng/dL
Male	6 Years	7 Years	Normal	Less than 13	ng/dL
Male	8 Years	9 Years	Normal	2 - 8	ng/dL
Male	10 Years	11 Years	Normal	2 - 165	ng/dL
Male	12 Years	13 Years	Normal	3 - 619	ng/dL
Male	14 Years	15 Years	Normal	31 - 733	ng/dL
Male	16 Years	17 Years	Normal	158 - 826	ng/dL
Male	18 Years	39 Years	Normal	300 - 1080	ng/dL
Male	40 Years	59 Years	Normal	300 - 890	ng/dL
Male	60 Years	99 Years	Normal	300 - 720	ng/dL
Testosterone, Bioavailable (LC-MS/MS)
Sex	Age From	Age To	Type	Range	Range Unit
Female			Normal	Postmenopausal: 1.5 - 9.4	ng/dL
Female			Normal	Tanner Stage I: 0.3 - 5.5	ng/dL
Female			Normal	Tanner Stage II: 1.2 - 15.0	ng/dL
Female			Normal	Tanner Stage III: 3.8 - 28.0	ng/dL
Female			Normal	Tanner Stage IV: 2.8 - 39.0	ng/dL
Female			Normal	Tanner Stage V: 2.5 - 23.0	ng/dL
Female	1 Years	6 Years	Normal	Less than 1.3	ng/dL
Female	7 Years	9 Years	Normal	0.3 - 5.0	ng/dL
Female	10 Years	11 Years	Normal	0.4 - 9.6	ng/dL
Female	12 Years	13 Years	Normal	1.7 - 18.8	ng/dL
Female	14 Years	15 Years	Normal	3.0 - 22.6	ng/dL
Female	16 Years	17 Years	Normal	3.3 - 28.6	ng/dL
Female	18 Years	30 Years	Normal	2.2 - 20.6	ng/dL
Female	31 Years	40 Years	Normal	4.1 - 25.5	ng/dL
Female	41 Years	51 Years	Normal	2.8 - 16.5	ng/dL
Male			Normal	Tanner Stage I: 0.3 - 13.0	ng/dL
Male			Normal	Tanner Stage II: 0.3 - 59.0	ng/dL
Male			Normal	Tanner Stage III: 1.9 - 296.0	ng/dL
Male			Normal	Tanner Stage IV: 40.0 - 485.0	ng/dL
Male			Normal	Tanner Stage V: 124.0 - 596.0	ng/dL
Male	1 Years	6 Years	Normal	Less than 1.3	ng/dL
Male	7 Years	9 Years	Normal	0.3 - 2.8	ng/dL
Male	10 Years	11 Years	Normal	0.1 - 17.9	ng/dL
Male	12 Years	13 Years	Normal	1.4 - 288.0	ng/dL
Male	14 Years	15 Years	Normal	9.5 - 337.0	ng/dL
Male	16 Years	17 Years	Normal	35.0 - 509.0	ng/dL
Male	18 Years	99 Years	Normal	130.0 - 680.0	ng/dL
Testosterone, Free
Sex	Age From	Age To	Type	Range	Range Unit
Female			Normal	Postmenopausal: 0.6 - 3.8	pg/mL
Female			Normal	Tanner Stage I: Less than 2.2	pg/mL
Female			Normal	Tanner Stage II: 0.4 - 4.5	pg/mL
Female			Normal	Tanner Stage III: 1.3 - 7.5	pg/mL
Female			Normal	Tanner Stage IV: 1.1 - 15.5	pg/mL
Female			Normal	Tanner Stage V: 0.8 - 9.2	pg/mL
Female	1 Years	6 Years	Normal	Less than 0.6	pg/mL
Female	7 Years	9 Years	Normal	0.6 - 1.8	pg/mL
Female	10 Years	11 Years	Normal	0.1 - 3.5	pg/mL
Female	12 Years	13 Years	Normal	0.9 - 6.8	pg/mL
Female	14 Years	15 Years	Normal	1.2 - 7.5	pg/mL
Female	16 Years	17 Years	Normal	1.2 - 9.9	pg/mL
Female	18 Years	30 Years	Normal	0.8 - 7.4	pg/mL
Female	31 Years	40 Years	Normal	1.3 - 9.2	pg/mL
Female	41 Years	51 Years	Normal	1.1 - 5.8	pg/mL
Male			Normal	Tanner Stage I: Less than or equal to 3.7	pg/mL
Male			Normal	Tanner Stage II: 0.3 - 21.0	pg/mL
Male			Normal	Tanner Stage III: 1.0 - 98.0	pg/mL
Male			Normal	Tanner Stage IV: 35.0 - 169.0	pg/mL
Male			Normal	Tanner Stage V: 41.0 - 239.0	pg/mL
Male	1 Years	6 Years	Normal	Less than 0.6	pg/mL
Male	7 Years	9 Years	Normal	0.1 - 0.9	pg/mL
Male	10 Years	11 Years	Normal	0.1 - 6.3	pg/mL
Male	12 Years	13 Years	Normal	0.5 - 98.0	pg/mL
Male	14 Years	15 Years	Normal	3.0 - 138.0	pg/mL
Male	16 Years	17 Years	Normal	38.0 - 173.0	pg/mL
Male	18 Years	99 Years	Normal	47.0 - 244.0	pg/mL

Special Info

Collection between 6 - 10 a.m. is preferred but not required.

Clinical Info

Bioavailable testosterone concentration is calculated using total testosterone (measured by mass spectrometry) and the binding constant of testosterone and sex hormone-binding globulin (SHBG) and/or albumin. 6 - 10 a.m. collection is preferable since the reference ranges are determined based on collections during that time. Results for patients collected outside 6 - 10 a.m. will be reported but must be interpreted in the context of the alternate collection time. Samples collected after 10 a.m. could have falsely low testosterone, and results should be interpreted carefully. For individuals on testosterone-suppressing hormone therapies (e.g., antiandrogens or estrogens), refer to cisgender female reference intervals.