Bordetella pertussis and parapertussis DNA, Nucleic Acid Amplification, Nasopharyngeal Swab
Test Mnemonic
BORAMP
CPT Codes
- 87798 - QTY (2)
LOINC ®
43913-3
Aliases
- Pertussis
- Whooping Cough
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 - 3 mL | Nasopharyngeal swab | Swab in Universal Transport Media (UTM) | Refrigerated | Acceptable specimen types include nasopharyngeal samples collected using flocked swabs in universal transport medium (UTM) or an equivalent viral transport medium. Example accepted collection kits include Oracle #1063581/1035694, Diagnostic Hybrids #402C/403C. Dry swabs, lower respiratory tract specimens (bronchoalveolar lavage, bronchial washings) or other upper respiratory specimens (sputum, throat swabs) will be rejected. Calcium-alginate swabs are not acceptable. |
Stability
| Environmental Condition | Description |
|---|---|
| Refrigerated | Specimens are stable up to 97 hours at 2-8C before testing. |
| Ambient | Specimens are stable up to 49 hours at 15-30C. |
| Frozen | Specimens are stable at up to 5 months at -70C. |
Days Performed
7 days a week
Turnaround Time
1 - 3 days
Methodology
| Name | Description |
|---|---|
| Amplification Detection | |
| Molecular | |
| Other |
Reference Range
Special Info
This test should not be used as a test of cure to monitor response to therapy.
Clinical Info
Symptoms of pertussis include coughing, sneezing, and runny nose. Within 1-2 weeks a paroxysmal cough followed by a "whoop" sound in infants and children or persistent cough in adolescents or adults develops. Testing should be performed within 4 weeks of cough onset.
Clinical Limitation
The Solana Bordetella Complete Assay is an HDA-based duplex assay that targets the IS481 and IS1001 sequence of Bordetella pertussis (BP) and Bordetella parapertussis (BPP) genomes, respectively. This sequence may also be found in other species of Bordetella. B. holmesii may cause clinical illness similar to B. pertussis and mixed outbreaks have been reported. B. bronchiseptica is a rare cause of human infection. Additional testing should be performed if needed to differentiate B. pertussis from B. holmesii.