Borrelia burgdorferi VlsE1/pepC10 Antibodies, CSF, Total by ELISA With Reflex to IgM and IgG by Immunoblot (Standard Two-Tier Testing, CSF)
Test Mnemonic
BBURGM
CPT Codes
- 86618 - QTY (1)
- if reflexed, add 86617 - QTY (2)
Aliases
- Lyme Antibodies, Total
Includes
- Lyme Standard 2-Tier, CSF, 1st Tier
- B. burgdorferi VlsE1/pepC10 Abs, CSF
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 6 mL | Cerebrospinal fluid (CSF) | Clean container | Refrigerated | Transfer 6 mL CSF to standard aliquot tube |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2.5 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 8 hours |
| Refrigerated | 2 weeks |
| Frozen | 1 month (Avoid repeated freeze/thaw cycles) |
Days Performed
Sun - Sat
Turnaround Time
2 - 5 days
Methodology
| Name | Description |
|---|---|
| Semi Quantitative Enzyme Linked Immunosorbent Assay |
Special Info
Heat-inactivated, contaminated, hemolyzed, or xanthochromic specimens are unacceptable.
Clinical Info
REFLEX CRITERIA: If VlsE1/pepC10 antibodies by ELISA is 0.91 IV or greater, then B. burgdorferi IgG antibody by immunoblot and IgM antibody by immunoblot will be added. Additional charges apply. If the patient is a New York state resident, please place this order as a Miscellaneous Test order (SQMISC1) for ARUP 3016760 and request 2 mL CSF and 2 mL serum (collected in a gold-top SST or red-top tube). ARUP will send the specimen to a New York DOH approved laboratory, if possible.