BRAF V600E Mutation Detection Other

Test Mnemonic


CPT Codes

  • 81210 - QTY (1)


  • Hairy cell leukemia BRAF V600E Other (paraffin embedded tissue)

Performing Laboratory

Cleveland Clinic Laboratories

Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
OneClotFormalin-fixed, paraffin-embedded blockAmbientAmbientParaffin- embedded clot should be delivered to Anatomic Pathology for accessioning and cutting.
10 mm squareParaffin block, formalin fixedClean containerAmbientAmbientParaffin- embedded tissue should be delivered to Anatomic Pathology for accessioning and cutting.


Environmental Condition Description
AmbientParaffin-embedded tissue- indefinitely; Paraffin-embedded clot- indefinitely
RefrigeratedParaffin-embedded tissue- unacceptable; Paraffin-embedded clot- unacceptable
FrozenParaffin-embedded tissue- unacceptable; Paraffin-embedded clot- unacceptable

Days Performed

3 days per week

Turnaround Time

5 days


Name Description
Droplet Digital Polymerase Chain Reaction (PCR) 

Special Info

May be reported with reduced sensitivity in cases of suboptimal amplification due to specimen type (with comment)

Clinical Info

This assay uses Droplet Digital PCR to detect the BRAF V600E mutation, which may be useful in hairy cell leukemia and in multiple other neoplasms including melanoma, colorectal carcinoma, papillary thyroid carcinoma, and Langerhans cell histiocytosis

Clinical Limitation

This test is designed to detect only the V600E variant in the BRAF gene. Other variants in BRAF will not be identified by this test. The lower limit of detection of this assay is approximately 0.1% variant allele fraction (vaf) for the BRAF V600E variant. The result cannot be used as a standalone basis for diagnosis of malignancy, and needs to be considered in the context of clinical and morphologic presentation.