Brucella abortus IgG, IgM




Test Mnemonic

BRUABS

CPT Codes

  • 86622 - QTY (2)

Performing Laboratory

Mayo Clinic Dpt of Lab Med & Pathology


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mLSerumSST (Gold) RefrigeratedCentrifuge and transfer serum into a standard plastic aliquot tube.

Alternate Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mLSerumNo additive (Red) RefrigeratedCentrifuge and transfer serum into a standard plastic aliquot tube.

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
0.4 mL     

Stability

Environmental Condition Description
AmbientUnacceptable
Refrigerated14 days (preferred)
Frozen14 days

Days Performed

Mon, Wed, Fri

Turnaround Time

2 - 4 days

Methodology

Name Description
Enzyme-Linked Immunosorbent Assay (ELISA) 

Reference Range

Special Info

If Brucella antibody screen IgM or IgG is reactive, then confirmation by Brucella total antibody agglutination testing will be performed at an additional cost, and this could delay turnaround time.

Clinical Info

To aid in diagnosis of brucellosis. Brucella canis, a rare cause of brucellosis, may not be detected by this method. This test utilizes antigen derived from Brucella abortus strain W99. However, significant cross-reactivity exists for other Brucella species (except B. canis), therefore, the assays should not be used to differentiate infection at the species level. Detection of specific IgM or IgG-class antibody to B. melitensis and B. suis by this method has not been determined.