Chromosome Analysis, Chorionic Villus
Test Mnemonic
CVCYTO
CPT Codes
- 88280 - QTY (1)
- 88267 - QTY (1)
- 88285 - QTY (1)
- 88235 - QTY (1)
Performing Laboratory
Integrated Genetics/Labcorp
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 30 mg | Chorionic Villus | RPMI media | Ambient | Do NOT freeze or place in fixative. Deliver specimen to Cleveland Clinic Laboratories on the day of collection. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 5 mg |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 5 days (Preferred) |
| Refrigerated | 5 days |
| Frozen | Unacceptable |
Days Performed
Mon - Sat
Turnaround Time
7 - 11 days
Methodology
| Name | Description |
|---|---|
| Giemsa-Band Analysis |
Reference Range
Special Info
Specimen is collected in syringe (transabdominal) or catheter (transcervical) and transferred by flushing with the media from the sterile container back into it. After collecting the specimen, wash with sterile saline solution (NaCl 0.95%) containing sodium heparin (two to three drops of sodium heparin in 10 mL of saline). Carefully transfer the specimen, into the CVS transport tube, using a sterile Pasteur pipette or a sterile fine needle forceps. Be sure to fill transport tubes completely with media. Samples from twin (multiple) pregnancies should be appropriately labeled and placed in separate transport containers with a separate request form for each twin. Do NOT freeze. Frozen specimens, specimens placed in fixative, improper labeling, and no villi submitted in specimen are unacceptable conditions.
Clinical Info
Chorionic villi sampling (CVS) chromosome analysis is recommended to be offered to all pregnant women by the American College of Obstetricians and Gynecologists (ACOG) for diagnostic testing (as opposed to just screening) for chromosome abnormalities. Additional specific indications for cytogenetic analysis include abnormal maternal serum screening results, abnormalities seen on ultrasound, family history of a chromosome abnormality, or known parental balanced chromosome rearrangement.