Estradiol-17 B




Test Mnemonic

E2

CPT Codes

  • 82670 - QTY (1)

LOINC ®

2243-4

Aliases

  • E2IVF

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mLPlasmaLithium heparin PST (Lt. Green) Centrifuge and refrigerate.Submit in original tube or aliquot into CCL aliquot tube.

Alternate Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mLSerumSST (Gold) Centrifuge and refrigerate. 

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
0.5 mL     

Stability

Environmental Condition Description
Ambient24 hours
Refrigerated2 days
Frozen6 months

Days Performed

Sun - Sat

Turnaround Time

8 hours

Methodology

Name Description
Electro Chemiluminescence Immunoassay (ECLIA) 

Reference Range

Estradiol-17 B
Sex Age From Age To Type Range Range Unit
Female      NormalSee Clinical Information 
Male      Normal< 38pg/mL

Special Info

Patients taking a biotin dose of up to 5 mg/day should refrain from taking biotin for 4 hours prior to sample collection. Patients taking a biotin dose of 5 to 10 mg/day should refrain from taking biotin for 8 hours prior to sample collection. Patients taking a biotin dose > 10 mg/day should consult with their physician or the laboratory prior to having a sample taken. Clinicians should consider biotin interference as a source of error, when clinically suspicious of the laboratory result.

Clinical Info

Differential diagnosis of amenorrhea; monitoring ovulation induction; gynecomastia; precocious puberty in females. Female Estradiol interpretive guidelines: Menstrual cycle Estradiol reference ranges: Follicular: < 234 pg/mL Ovulation: 41 to 398 pg/mL Luteal: < 342 pg/mL Pregnancy Estradiol reference ranges vary by gestational period: First trimester: 154 to 3243 pg/mL Second trimester: 1561 to 21280 pg/mL Third trimester: 8285 to >30000 pg/mL Post-menopausal Estradiol: < 41 pg/mL Reference: 1. Estradiol – E2 (Estradiol III) [package insert V 3.0 English]. Roche Diagnostics, Indianapolis, IN; June 2016

Clinical Limitation

This test is not suitable for patients receiving treatment with the drug Fulvestrant (Faslodex). The drug causes an interference leading to falsely elevated estradiol results.