Fecal Occult Blood Test

Test Mnemonic


CPT Codes

  • 82274 - QTY (1)




  • Colon Cancer Screen

Performing Laboratory

Cleveland Clinic Laboratories

Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
UnspecifiedStoolOther AmbientThe only acceptable specimen is stool inoculated into a Polymedco sample collection vial. Bulk stool (stool not contained in a Polymedco collection vial) will be rejected. Record date and time of collection on the test vial. Patients should be instructed to place the inoculated test vial into the preaddressed mailer along with a copy of the order and mail to microbiology.


Environmental Condition Description
AmbientInoculated collection vials are stable for up to 15 days
RefrigeratedInoculated collection vials are stable up to 30 days at 4 degrees Centigrade

Days Performed

Mon - Fri

Turnaround Time

4 days


Name Description
Immunoassay (IA) 

Reference Range

Special Info

Acceptable specimens include formed or semiformed stool. No dietary or pharmaceutical restrictions are necessary. Other specimens (such as gastric aspirates and other body fluids) are unacceptable. Conditions which may interfere with test results include bleeding due to hemorrhoids, constipation, urinary or menstrual sources. Avoid contamination of the sample with toilet water. Stool must be inoculated into the collection vial prior to sending to the lab for testing. Bulk stool will not be accepted due to the instability of blood in bulk stool. To inoculate the collection vial, remove the GREEN cap from the vial and scrape the fecal sample with the attached sample probe. Cover the grooved portion of the sample probe completely with stool. Return the green cap to the sample vial, tighten cap and mix well. Place a barcoded label onto the outside of the sample vial. The label needs to be placed so that the barcode is visible on the flat side of the sample vial. The label needs to be centered and aligned as straight as possible. DO NOT COVER THE FOIL EDGE OR GREEN CAP OF THE TUBE. Submit to microbiology for testing.

Clinical Info

This IFOBT replaces the Hemoccult triple card and is designed for detection of colon cancer in the lower GI. The IFOBT is designed to detect hemoglobin in stool as low as 100 ng/mL so that only a single sample is recommended for testing. This test does not detect blood in the upper GI. To test for upper or lower GI occult blood, please order the OBDX and inoculate a single Hemoccult or Hemaprompt card. Use of Hemaprompt test cards is limited to authorized users only who have received the appropriate credentialing from the POCT office.

Technical Brief