FISH for Aggressive B-cell Lymphoma

Test Mnemonic


CPT Codes

  • 88377 - QTY (4)


  • FISH for Aggressive B-Cell Lymphoma (Tissue)


  • FISH for BCL2 Translocations
  • FISH for BCL6
  • FISH for c-MYC (8q24)
  • FISH for t(8;14)(q24;32)

Performing Laboratory

Cleveland Clinic Laboratories

Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
6Slide (s), unstainedClean containerAmbientAmbientOne formalin-fixed, paraffin embedded block containing representative tissue OR unstained 4 μm tissue sections on electrostatically charged slides containing representative tumor. Tissue must be fixed in formalin NOT B5 or PREFER fixative


Environmental Condition Description
AmbientSample is formalin fixed and must not be fixed in Prefer or B5

Days Performed

5 days per week

Turnaround Time

3 - 5 days


Name Description
Fluorescent In-Situ Hybridization (FISH) 

Clinical Info

Interphase FISH analysis used to assist in the diagnosis of various aggressive B-cell lymphomas. A panel of four FISH probes are employed: a dual color MYC break-apart probe, a dual fusion, dual color MYC::IGH probe, a dual color BCL2 break-apart probe, and a dual color BCL6 break-apart probe. The translocation partner gene(s) cannot be identified in this assay. If no cells are available for analysis, no analysis charges will be incurred.