FISH for Ewings Sarcoma
Test Mnemonic
EWSR
CPT Codes
- 88377 - QTY (1)
Aliases
- Ewing Sarcoma Gene (22q12) translocation
- Fluorescence in Situ Hybridization for Ewings Sarc
Includes
- LSI 22q12
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 slides | Slide (s), unstained | Clean container | Ambient | One formalin-fixed, paraffin embedded block containing representative tissue OR unstained 4 μm tissue sections on electrostatically charged slides containing representative tumor. Tissue must be fixed in formalin NOT B5 or PREFER fixative |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Slides/Blocks stable at ambient temperatures. |
Days Performed
5 days per week
Turnaround Time
5 - 7 days
Methodology
| Name | Description |
|---|---|
| Fluorescent In-Situ Hybridization (FISH) |
Clinical Info
Interphase FISH analysis utilizing a dual color, break-apart rearrangement probe for the EWSR1 gene at 22q12.2. Rearrangements involving the EWSR1 gene are observed in various soft tissue tumors, including Ewing sarcoma and desmoplastic small round cell tumor. The translocation partner gene cannot be identified in this assay. If no cells are available for analysis, no analysis charges will be incurred.