FLT3 ITD and TKD Mutation Detection by PCR
Test Mnemonic
FLT3IT
CPT Codes
- 81245 - QTY (1)
- 81246 - QTY (1)
Includes
- FLT3 Source
- ITD Result
- ITD Ratio
- TKD Result
- FLT3 ITD and TKD Mutation Detection
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 5 mL | Whole blood | EDTA (Lavender) | Refrigerated | Do NOT freeze. | |
| 3 mL | Bone marrow | EDTA (Lavender) | Refrigerated | Do NOT freeze. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| N/A | Extracted DNA | See note | Refrigerated | Transport 40 uL DNA with a concentration of at least 50 ng/uL (minimum 40 uL) using a tissue transport kit (ARUP supply #47808). DNA must be extracted by a CLIA certified lab. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Blood or bone marrow |
Stability
| Environmental Condition | Description |
|---|---|
| Refrigerated | Blood, bone marrow: 5 days; Extracted DNA: Indefinitely |
| Ambient | Blood, bone marrow: 24 hours; Extracted DNA: 1 month |
| Frozen | Blood, bone marrow: Unacceptable; Extracted DNA: Indefinitely |
Days Performed
Varies
Turnaround Time
3 - 8 days
Methodology
| Name | Description |
|---|---|
| Polymerase Chain Reaction (PCR) |
Reference Range
Special Info
DNA isolation is performed Sunday - Saturday. Plasma, serum, FFPE tissue blocks/slides, or frozen tissue will be rejected. Specimens collected in anticoagulants other than EDTA or sodium heparin are unacceptable. Clotted or grossly hemolyzed specimens will be rejected. This test is New York DOH approved.
Clinical Info
Aids in diagnosis and management of acute myeloid leukemia. Not intended for minimal residual disease monitoring.