Haemophilus influenzae B Ab IgG
Test Mnemonic
HINFLU
CPT Codes
- 86317 - QTY (1)
Aliases
- H Flu B
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Serum | SST (Gold) | Refrigerated | Separate serum from cells ASAP or within 2 hours of collection and transfer into standard aliquot tube. "Pre" and 30-day "post" Haemophilus influenzae b vaccination specimens should be submitted together. "Post" specimen should be drawn 30 days after immunization and must be received within 60 days of "pre" specimen. Label specimens clearly as "Pre-Vaccine" or "Post-Vaccine." |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.15 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Refrigerated | After separation from cells: 2 weeks |
| Frozen | After separation from cells: 1 year (avoid repeated freeze/thaw cycles) |
| Ambient | After separation from cells: 48 hours |
Days Performed
Sun - Sat
Turnaround Time
2 - 4 days
Methodology
| Name | Description |
|---|---|
| Quantitative Multiplex Bead Assay |
Reference Range
| Haemophilus influenzae B Ab IgG | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| < 1.0 ug/mL = Antibody concentration not protective | Greater than or equal to 1.0 ug/mL = Antibody to H. influenzae b detected; Suggestive of protection |
Special Info
Plasma or other body fluids are unacceptable. Contaminated, hemolyzed, or severely lipemic specimens will not be accepted. This test is New York DOH approved.
Clinical Info
Evaluate the ability of a patient to produce antibody to a protein conjugated bacterial (H. influenza) vaccine to rule out antibody deficiency. Responder status is determined according to the ratio of post-vaccination concentration to pre-vaccination concentration of Haemophilus influenza b antibody, IgG as follows: 1. If the post-vaccination concentration is less than 3.0 µg/mL, the patient is considered to be a nonresponder. 2. If the post-vaccination concentration is greater than or equal to 3.0 µg/mL, a patient with a ratio of greater than or equal to 4 is a good responder, a ratio of 2-4 is a weak responder, and a ratio of less than 2 is considered a nonresponder.