Hepatitis B Virus DNA Quantification
Test Mnemonic
HBVDNU
CPT Codes
- 87517 - QTY (1)
Aliases
- HBV DNA Detection
- Hepatitis B DNA Viral Load
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 mL | Plasma | EDTA PPT (White) | Refrigerated | Separate plasma from red cells within 24 hours of collection. If aliquoting is necessary, sterile aliquot tubes must be used. Sample must be aliquoted first if sample is to be frozen. Sample cannot be shared with a test performed outside of Molecular Microbiology. Sample can only be shared with CMVQNT, HCQPCR, HIVRNA, HBVDNU, EBVQNT, or BKQUAN. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 mL | Plasma | EDTA (Lavender) | Refrigerated | Separate plasma from red cells within 24 hours of collection. Sterile aliquot tubes must be used. Sample must be aliquoted first if sample is to be frozen. Sample cannot be shared with a test performed outside of Molecular Microbiology. Sample can only be shared with CMVQNT, HCQPCR, HIVRNA, HBVDNU, EBVQNT, or BKQUAN. | |
| 2 mL | Serum | SST (Gold) | Refrigerated | Separate serum from red cells within 24 hours of collection. If aliquoting is necessary, sterile aliquot tubes must be used. Sample must be aliquoted first if sample is to be frozen. Sample cannot be shared with a test performed outside of Molecular Microbiology. Sample can only be shared with CMVQNT, HCQPCR, HIVRNA, HBVDNU, EBVQNT, or BKQUAN. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1.5 mL | If aliquoting is necessary, sterile aliquot tubes must be used. Sample must be aliquoted first if sample is to be frozen. Sample cannot be shared with a test performed outside of Molecular Microbiology. Sample can only be shared with CMVQNT, HCQPCR, HIVRNA, HBVDNU, EBVQNT, or BKQUAN. |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 24 hours |
| Refrigerated | 6 days |
| Frozen | 12 weeks at <-18°C |
Days Performed
Varies, Mon - Fri
Turnaround Time
3 - 5 days
Methodology
| Name | Description |
|---|---|
| Polymerase Chain Reaction (PCR) |
Reference Range
| Hepatitis B Viral DNA, Ultra Quant | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Negative for HBV DNA |
Special Info
The quantitative range of this assay is 10 IU/mL to 1,000,000,000 IU/mL. Elevated levels of hemoglobin at concentrations of 500 mg/dl may interfere with the quantitation. Rare mutations in the highly conserved region of the viral genome may cause under-quantitation or failure to detect the virus.
Clinical Info
Quantitation of HBV-DNA in serum or plasma is used for the management of chronic HBV infection, including monitoring anti-viral therapy.