Hepatitis B Virus Genotyping
Test Mnemonic
HBVGEN
CPT Codes
- 87912 - QTY (1)
Includes
- Hepatitis B Genotype
- HBV Surface Antigen Mutations
- HBV RT Polymerase Mutations
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 mL | Serum | SST (Gold) | Frozen | Separate serum from cells within 24 hours. Transfer 2 mL serum to standard aliquot tube. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 mL | Plasma | EDTA (Lavender) | Frozen | Separate plasma from cells within 24 hours. Transfer 2 mL plasma to standard aliquot tube. | |
| 2 mL | Plasma | EDTA PPT (White) | Frozen | Separate plasma from cells within 24 hours. Transfer 2 mL plasma to standard aliquot tube. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.5 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | After separation from cells: 72 hours |
| Refrigerated | After separation from cells: 1 week |
| Frozen | After separation from cells: 6 weeks |
Days Performed
Tue, Fri
Turnaround Time
11 - 12 days
Methodology
| Name | Description |
|---|---|
| Polymerase Chain Reaction (PCR) | |
| Sequencing |
Special Info
Please submit most recent viral load and test date, if available. This test may be unsuccessful if the HBV viral load is less than log 3.0 or 1,000 IU/ml. of plasma. Heparinized specimens are unacceptable. This test is New York DOH approved.
Clinical Info
Determine antiviral drug resistance by DNA sequencing. Both the HBV RT polymerase and the HBsAg encoding regions are sequenced. Resistance and surface antigen mutations are reported. In addition, the major HBV genotypes are identified. Mutations in viral sub-populations below 20% of total may not be detected.