Histoplasma capsulatum Antigen
Test Mnemonic
SHISTO
CPT Codes
- 87385 - QTY (1)
Aliases
- Histoplasma Antigen Quantitative EIA
Includes
- Histoplasma Ag
Performing Laboratory
MiraVista Diagnostics
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 mL | Serum | SST (Gold) | Refrigerated | Allow blood to clot for 30 minutes, then centrifuge. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 mL | Bronch (BAL) | Sterile container | Refrigerated | ||
| 2 mL | Cerebrospinal fluid (CSF) | Sterile container | Refrigerated | ||
| 2 mL | Fluid, body | Sterile container | Refrigerated | ||
| 2 mL | Serum | No additive (Red) | Refrigerated | Allow blood to clot for 30 minutes, then centrifuge. | |
| 2 mL | Plasma | EDTA (Lavender) | Refrigerated | ||
| 2 mL | Plasma | Sodium or Lithium heparin (Green) | Refrigerated |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1.2 mL | Serum/Plasma | ||||
| 0.8 mL | CSF Only | ||||
| 0.5 mL | BAL and other body fluids |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 2 weeks |
| Refrigerated | 2 weeks |
| Frozen | Indefinitely |
Days Performed
Mon - Sat
Turnaround Time
2 - 4 days
Methodology
| Name | Description |
|---|---|
| Enzyme Immunoassay (EIA) |
Reference Range
Special Info
Specimens too viscous to pipette will be rejected. Tissue, FNA, Biopsy, sputum, bronchial brushings, stool, bone marrow and tracheal aspirate specimens will also be rejected. Serum and CSF are pre-treated to improve sensitivity. Sputolysin, sodium hydroxide and potassium hydroxide degrade the analyte detected in the assay.
Clinical Info
REPORTABLE RANGE: 2ng/mL – 20.0ng/mL; Results above 20.0ng/mL are reported as Positive, Above the Limit of Quantification •This test is useful in diagnosing and monitoring treatment of histoplasmosis. Cross-reactivity can occur with very closely related fungi such as Blastomyces, Coccidioides, Talaromyces, and Paracoccidioides, causing false-positive Histoplasma antigen results. Clinically, differentiation of these organisms might not be necessary as treatment is the same.