HIV 1 Drug Resistance by Next Generation Sequencing




Test Mnemonic

HIVNGS

CPT Codes

  • 87900 - QTY (1)
  • 87906 - QTY (1)
  • 87901 - QTY (1)

Includes

  • HIV-1 Drug Resistance by NGS
  • EER HIV-1 Drug Resistance by NGS

Performing Laboratory

ARUP


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
3 mLPlasmaEDTA (Lavender) FrozenSeparate plasma from cells within 24 hours and transfer plasma to a standard aliquot tube. Please submit most recent viral load and test date, if available.

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2.5 mL     

Stability

Environmental Condition Description
FrozenAfter separation from cells: 3 months
RefrigeratedAfter separation from cells: 72 hours
AmbientAfter separation from cells: 24 hours

Days Performed

Sun - Sat

Turnaround Time

5 - 11 days

Methodology

Name Description
Massive Parallel Sequencing 

Reference Range

ERR HIV1 Drug Resistance
Sex Age From Age To Type Range Range Unit
       Refer to Report 
HIV1 Drug Res by NGS
Sex Age From Age To Type Range Range Unit
       Refer to Report 

Special Info

Please submit most recent viral load and test date, if available. Serum and heparinized specimens are unacceptable. This test is New York State approved.

Clinical Info

This assay predicts HIV-1 resistance to protease inhibitors, nucleoside reverse transcriptase inhibitors, non-nucleoside reverse transcriptase inhibitors and integrase inhibitors. The protease gene, integrase gene and the reverse transcriptase gene of the viral genome are sequenced using Next Generation Sequencing. Drug resistance is assigned using the Stanford hivdb database. This test should be used in conjunction with clinical presentation and other laboratory markers. A patient's response to therapy depends on multiple factors, including patient adherence, percentage of resistant virus population, dosing, and drug pharmacology issues.

Clinical Limitation

This test detects populations down to 10 percent of the total population which may account for resistance interpretation differences between methods. Some insertions or deletions may be difficult to detect using this software. This test may be unsuccessful if the plasma HIV-1 RNA viral load is less than 500 copies per mL of plasma.