HIV Quant RNA by PCR
Test Mnemonic
HIVRNA
CPT Codes
- 87536 - QTY (1)
LOINC ®
20447-9
Aliases
- HIV RNA
- HIV RNA
- HIV RNA Detection Quantitative
- HIV RNA Detection Quantitative
- HIV Viral Load
- HIV Viral Load
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 3 mL | Plasma | EDTA PPT (White) | Refrigerated | Centrifuge within 24 hours of collection. Sample must be aliquoted first if sample is to be frozen. Sample can only be shared with CMVQNT, HCQPCR, HIVRNA, HBVDNU, EBVQNT, or BKQUAN. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 3 mL | Plasma | EDTA (Lavender) | Refrigerated | Separate plasma from whole blood within 24 hours of collection by centrifugation and transfer to a sterile screw cap polypropylene tube. Sample can only be shared with CMVQNT, HCQPCR, HIVRNA, HBVDNU, EBVQNT, or BKQUAN. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 3 mL | Sample can only be shared with CMVQNT, HCQPCR, HIVRNA, HBVDNU, EBVQNT, or BKQUAN. |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Plasma: 24 hours |
| Refrigerated | Plasma: 6 days |
| Frozen | Plasma: 6 months (Plasma cannot be frozen in PPT tube.) |
Days Performed
Varies, Mon - Fri
Turnaround Time
3 - 5 days
Methodology
| Name | Description |
|---|---|
| Polymerase Chain Reaction (PCR) |
Reference Range
| HIV Quant RNA by PCR | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Negative for HIV-1 RNA |
Special Info
EDTA PPT (White) - Centrifuge within 24 hrs of collection. Do not aliquot.
Clinical Info
This test is intended for use in conjunction with clinical presentation and as an indication of disease prognosis in the clinical management of HIV-1 infected patients.The test can be used to establish a baseline level and/or to monitor antiretroviral therapy. The linear range of this assay is 20 copies/mL to 10,000,000 copies/mL. The test quantitates all major Group M subtypes A-H, including circulating recombinant forms and HIV-1 group O virus. The LTR and the gag region of the HIV-1 genome are amplified and detected.