HSV PCR - Miscellaneous Specimen Types
Test Mnemonic
PCRHSV
CPT Codes
- 87529 - QTY (2)
Aliases
- Herpes Simplex Virus by PCR
Includes
- HSV 1 Subtype by PCR
- HSV 2 Subtype by PCR
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Plasma | EDTA (Lavender) | Frozen | Separate plasma from cells and transfer into sterile aliquot tube. Specimen source required. | |
| 1 mL | Serum | SST (Gold) | Frozen | Separate serum from cells and transfer into sterile aliquot tube. Specimen source required. | |
| 1 mL | Fluid, amniotic | Sterile container | Frozen | Specimen source required. | |
| 1 mL | Bronch (BAL) | Sterile container | Frozen | Specimen source required. | |
| N/A | Tissue | Sterile container | Frozen | Transfer tissue to sterile container and freeze immediately. Specimen source required. Do not send tissues in optimal cutting temperature compound. | |
| 1 mL | Fluid, ocular | Sterile container | Frozen | Specimen source required. Testing from ocular fluid may be performed with a disclaimer for short volume on as little as 20 uL sample. The performing laboratory (ARUP) will determine whether there is sufficient volume for testing to be performed. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 3 mL | See note | Viral Transport Media | Frozen | Transfer vesicle fluid to Viral Transport Media. Specimen source required. | |
| One | Endocervical | Thin Prep | Frozen | Specimen source required. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.5 mL | Specimen source required. Testing from ocular fluid may be performed with a disclaimer for short volume on as little as 20 uL sample. The performing laboratory (ARUP) will determine whether there is sufficient volume for testing to be performed. |
Stability
| Environmental Condition | Description |
|---|---|
| Frozen | 3 months |
| Refrigerated | Tissue: Unacceptable; Plasma, serum, amniotic fluid, BAL, ocular fluid, vesicle fluid, Thin Prep: 72 hours |
| Ambient | Tissue: Unacceptable; Plasma, serum, amniotic fluid, BAL, ocular fluid, vesicle fluid, Thin Prep: 8 hours |
Days Performed
Sun - Sat
Turnaround Time
2 - 4 days
Methodology
| Name | Description |
|---|---|
| Qualitative Polymerase Chain Reaction |
Reference Range
Special Info
Specimen source is required. Heparinized specimens and tissues in optimal cutting temperature compound are unacceptable. This test is New York DOH approved.
Clinical Info
Genotype herpes simplex virus (HSV) types 1 and 2. A negative result does not rule out the presence of PCR inhibitors in the patient specimen or test-specific nucleic acid in concentrations below the level of detection by this test.